Overview

  • Product name

    Anti-HIV1 Rev antibody [Rev-6]
    See all HIV1 Rev primary antibodies
  • Description

    Mouse monoclonal [Rev-6] to HIV1 Rev
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    HIV1 Rev recombinant protein (clade B, HXB-3 isolate) with an N terminal His tag, expressed in bacteria.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 2.94% Sodium citrate, 0.05% Glycerol, 0.00061% Tris
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from supernatants of hybridoma cell cultures by affinity chromatography based on Protein A.
  • Clonality

    Monoclonal
  • Clone number

    Rev-6
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 13 kDa.
ELISA 1/100 - 1/200.

Target

  • Relevance

    HIV1 expresses the trans-activator protein Rev which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. The HIV1 Rev protein as viral protein is expressed early in the virus life cycle and thus may be important targets for the immune control of HIV1 infection.
  • Alternative names

    • Human immunodeficiency virus 1 Rev antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • Rev antibody

Images

  • Representative western blot detecting HIV1 Rev using ab85529 at 1/250 dilution. An HRP-conjugated secondary antibody (1/5000) was used.

    HeLa cells stably transduced with an inducible Tet-On HIV-1 system, were untreated or treated for 4-5 h with TG009 or chlorhexidine, followed by induction of expression of the endogenous HIV-1 provirus by addition of doxycycline.

References

This product has been referenced in:

  • Kyei GB  et al. Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency. MBio 9:N/A (2018). Read more (PubMed: 30401776) »
  • Behrens RT  et al. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export. J Virol 91:N/A (2017). Read more (PubMed: 27852860) »
See all 6 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 transfected with HIV-1Rev expression plasmi)
Loading amount
10 µg
Specification
HEK293 transfected with HIV-1Rev expression plasmi
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

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Verified customer

Submitted Jan 08 2013

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