Key features and details
- Mouse monoclonal [Rev-6] to HIV1 Rev
- Suitable for: WB, ELISA
- Isotype: IgG1
Product nameAnti-HIV1 Rev antibody [Rev-6]
See all HIV1 Rev primary antibodies
DescriptionMouse monoclonal [Rev-6] to HIV1 Rev
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Other species
HIV1 Rev recombinant protein (clade B, HXB-3 isolate) with an N terminal His tag, expressed in bacteria.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituents: 2.94% Sodium citrate, 0.05% Glycerol, 0.00061% Tris
Concentration information loading...
PurityProtein A purified
Purification notesPurified from supernatants of hybridoma cell cultures by affinity chromatography based on Protein A.
Light chain typekappa
Our Abpromise guarantee covers the use of ab85529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500. Predicted molecular weight: 13 kDa.|
|ELISA||1/100 - 1/200.|
RelevanceHIV1 expresses the trans-activator protein Rev which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. The HIV1 Rev protein as viral protein is expressed early in the virus life cycle and thus may be important targets for the immune control of HIV1 infection.
- Human immunodeficiency virus 1 Rev antibody
- Protein Rev antibody
- Regulator of expression of viral proteins antibody
- Rev antibody
Representative western blot detecting HIV1 Rev using ab85529 at 1/250 dilution. An HRP-conjugated secondary antibody (1/5000) was used.
HeLa cells stably transduced with an inducible Tet-On HIV-1 system, were untreated or treated for 4-5 h with TG009 or chlorhexidine, followed by induction of expression of the endogenous HIV-1 provirus by addition of doxycycline.
ab85529 has been referenced in 6 publications.
- Kyei GB et al. Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency. MBio 9:N/A (2018). PubMed: 30401776
- Behrens RT et al. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export. J Virol 91:N/A (2017). PubMed: 27852860
- Wong RW et al. Digoxin suppresses HIV-1 replication by altering viral RNA processing. PLoS Pathog 9:e1003241 (2013). WB . PubMed: 23555254
- Wong RW et al. Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function. Nucleic Acids Res 41:9471-83 (2013). Human . PubMed: 23945945
- Lund N et al. Differential effects of hnRNP D/AUF1 isoforms on HIV-1 gene expression. Nucleic Acids Res 40:3663-75 (2012). WB . PubMed: 22187150
- Wong R et al. Differential effect of CLK SR Kinases on HIV-1 gene expression: potential novel targets for therapy. Retrovirology 8:47 (2011). WB ; Human . PubMed: 21682887