Product nameAnti-HIV1 Reverse Transcriptase antibody [39/4.12.2]
See all HIV1 Reverse Transcriptase primary antibodies
DescriptionMouse monoclonal [39/4.12.2] to HIV1 Reverse Transcriptase
SpecificityThis antibody reacts with pol-encoded p51/p66 using Western blotting. Cross reacts with HIV2. No reactivity with HTLV I reverse transcriptase has been detected.
Tested applicationsSuitable for: ELISA, WBmore details
Unsuitable for: ICC/IF or IP
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Concentration information loading...
PurityProtein A purified
Purification notesPurified from serum-free culture supernatant.
Our Abpromise guarantee covers the use of ab9066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent concentration.|
RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
- HIV 1 Reverse transcriptase antibody
- HIV1 RT antibody
- Human Immunodeficiency virus 1 antibody
This product has been referenced in:
- Liang Y et al. Aptamer beacons for visualization of endogenous protein HIV-1 reverse transcriptase in living cells. Biosens Bioelectron 28:270-6 (2011). ICC/IF . Read more (PubMed: 21824761) »
- Iordanskiy S et al. Subtype-associated differences in HIV-1 reverse transcription affect the viral replication. Retrovirology 7:85 (2010). WB . Read more (PubMed: 20939905) »