Anti-HIV1 Reverse Transcriptase antibody [39/4.12.2] (ab9066)


  • Product name
    Anti-HIV1 Reverse Transcriptase antibody [39/4.12.2]
    See all HIV1 Reverse Transcriptase primary antibodies
  • Description
    Mouse monoclonal [39/4.12.2] to HIV1 Reverse Transcriptase
  • Host species
  • Specificity
    This antibody reacts with pol-encoded p51/p66 using Western blotting. Cross reacts with HIV2. No reactivity with HTLV I reverse transcriptase has been detected.
  • Tested applications
    Suitable for: ELISA, WBmore details
    Unsuitable for: ICC/IF or IP



Our Abpromise guarantee covers the use of ab9066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Relevance
      HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
    • Alternative names
      • HIV 1 Reverse transcriptase antibody
      • HIV1 RT antibody
      • Human Immunodeficiency virus 1 antibody
      • p51 RT antibody
      • p66 RT antibody
      • Reverse transcriptase p51 subunit antibody
      • Reverse transcriptase p66 subunit antibody
      see all


    This product has been referenced in:
    • Liang Y  et al. Aptamer beacons for visualization of endogenous protein HIV-1 reverse transcriptase in living cells. Biosens Bioelectron 28:270-6 (2011). ICC/IF . Read more (PubMed: 21824761) »
    • Iordanskiy S  et al. Subtype-associated differences in HIV-1 reverse transcription affect the viral replication. Retrovirology 7:85 (2010). WB . Read more (PubMed: 20939905) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry. I'm sorry, but we do not have data supporting which strain of HIV this antibody recognizes. Being an RT antibody, it may recognize some strains and not others. My apologies for the inconvenience,

    Thank you for your enquiry. We regret to inform you that we have not sequenced this antibody. It will cross react with HIV-2 and reacts with p66 and p51. We hope this helps.

    Thank you for your enquiry. The originator of this antibody has informed me that ab9066 has only been tested for application in Western blotting and has not been used in Enzyme Immunoassay. I have updated the online datasheet to reflect this and I apol...

    Read More


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