Key features and details
- Rabbit polyclonal to HIV1 Reverse Transcriptase
- Suitable for: WB, ELISA
- Isotype: IgG
Product nameAnti-HIV1 Reverse Transcriptase antibody
See all HIV1 Reverse Transcriptase primary antibodies
DescriptionRabbit polyclonal to HIV1 Reverse Transcriptase
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Other species
HIV1 Reverse Transcriptase, over-expressed as recombinant protein in E.coli
- Extract of MT4 cells infected with HIV1 (LAI strain).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab63911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500. Detects a band of approximately 51, 66 kDa.|
|ELISA||Use at an assay dependent dilution.|
RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
- HIV 1 Reverse transcriptase antibody
- HIV1 RT antibody
- Human Immunodeficiency virus 1 antibody
All lanes : Anti-HIV1 Reverse Transcriptase antibody (ab63911) at 1/2500 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)
Observed band size: 51,66 kDa why is the actual band size different from the predicted?
ab63911 has been referenced in 4 publications.
- Takahata T et al. Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse Transcriptase. J Virol 91:N/A (2017). PubMed: 27795445
- Garcia-Miranda P et al. Stability of HIV Frameshift Site RNA Correlates with Frameshift Efficiency and Decreased Virus Infectivity. J Virol 90:6906-17 (2016). PubMed: 27194769
- Leng J et al. A cell-intrinsic inhibitor of HIV-1 reverse transcription in CD4(+) T cells from elite controllers. Cell Host Microbe 15:717-728 (2014). PubMed: 24922574
- Contreras X et al. Protein kinase C-delta regulates HIV-1 replication at an early post-entry step in macrophages. Retrovirology 9:37 (2012). WB . PubMed: 22554282