Product nameAnti-HIV1 Reverse Transcriptase antibody
See all HIV1 Reverse Transcriptase primary antibodies
DescriptionGuinea pig polyclonal to HIV1 Reverse Transcriptase
Host speciesGuinea pig
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Other species
HIV1 Reverse Transcriptase, over-expressed as recombinant protein in E.coli.
- Extract of MT4 cells infected with HIV-1 (LAI strain).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab63912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500. Detects a band of approximately 51, 66 kDa.|
|ELISA||Use at an assay dependent concentration.|
RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
- HIV 1 Reverse transcriptase antibody
- HIV1 RT antibody
- Human Immunodeficiency virus 1 antibody
All lanes : Anti-HIV1 Reverse Transcriptase antibody (ab63912) at 1/2500 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)
Observed band size: 51,66 kDa why is the actual band size different from the predicted?
ab63912 detects bands of 66 kDa and 51 kDa corresponding to the two subunits of HIV1 reverse transcriptase.
ab63912 has not yet been referenced specifically in any publications.