Anti-HIV1 tat antibody [1] (ab42359)
Key features and details
- Mouse monoclonal [1] to HIV1 tat
- Suitable for: Radioimmunoprecipitation, IHC-P, ELISA, WB
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 tat antibody [1]
See all HIV1 tat primary antibodies -
Description
Mouse monoclonal [1] to HIV1 tat -
Host species
Mouse -
Specificity
This antibody reacts with the rTat consensus sequences of HIV1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE). -
Tested applications
Suitable for: Radioimmunoprecipitation, IHC-P, ELISA, WBmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Recombinant fragment corresponding to HIV-1 HIV1 tat.
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Epitope
Mapped to amino acids 2-9 (EPVDPRLE - B subtype consensus). -
General notes
Also known as clone 02-001.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Radioimmunoprecipitation |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
Use a concentration of 0.02 - 0.05 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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Radioimmunoprecipitation
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ELISA
Use a concentration of 0.02 - 0.05 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. |
Target
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Relevance
HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR. -
Cellular localization
Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region. -
Database links
- Entrez Gene: 155871 Human immunodeficiency virus
- SwissProt: P04608 Human immunodeficiency virus
- SwissProt: P04610 Human immunodeficiency virus
- SwissProt: Q6UFJ9 Human immunodeficiency virus
- SwissProt: Q900K4 Human immunodeficiency virus
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Alternative names
- p14 antibody
- Protein Tat antibody
- Transactivating regulatory protein antibody
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab42359 has been referenced in 11 publications.
- Lai M et al. CRISPR/Cas9 Ablation of Integrated HIV-1 Accumulates Proviral DNA Circles with Reformed Long Terminal Repeats. J Virol 95:e0135821 (2021). PubMed: 34549986
- Pereira LA et al. MYB elongation is regulated by the nucleic acid binding of NF?B p50 to the intronic stem-loop region. PLoS One 10:e0122919 (2015). PubMed: 25853889
- Vandergaast R et al. Generation of West Nile virus infectious clones containing amino acid insertions between capsid and capsid anchor. Viruses 6:1637-53 (2014). WB . PubMed: 24721788
- Lai MC et al. Human DDX3 interacts with the HIV-1 Tat protein to facilitate viral mRNA translation. PLoS One 8:e68665 (2013). IP . PubMed: 23840900
- Wright CA et al. Effects of Tat proteins and Tat mutants of different human immunodeficiency virus type 1 clades on glial JC virus early and late gene transcription. J Gen Virol 94:514-23 (2013). PubMed: 23152365