Key features and details
- Mouse monoclonal  to HIV1 tat
- Suitable for: Radioimmunoprecipitation, IHC-P, ELISA, WB
- Isotype: IgG1
Product nameAnti-HIV1 tat antibody 
See all HIV1 tat primary antibodies
DescriptionMouse monoclonal  to HIV1 tat
SpecificityThis antibody reacts with the rTat consensus sequences of HIV1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE).
Tested applicationsSuitable for: Radioimmunoprecipitation, IHC-P, ELISA, WBmore details
Species reactivityReacts with: Other species
Recombinant fragment corresponding to HIV-1 HIV1 tat.
EpitopeMapped to amino acids 2-9 (EPVDPRLE - B subtype consensus).
General notesAlso known as clone 02-001.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab42359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Radioimmunoprecipitation||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||1/500 - 1/2000.|
|WB||1/500 - 1/2000.|
RelevanceHIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
Cellular localizationNuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
- p14 antibody
- Protein Tat antibody
- Transactivating regulatory protein antibody
ab42359 has been referenced in 10 publications.
- Pereira LA et al. MYB elongation is regulated by the nucleic acid binding of NF?B p50 to the intronic stem-loop region. PLoS One 10:e0122919 (2015). PubMed: 25853889
- Vandergaast R et al. Generation of West Nile virus infectious clones containing amino acid insertions between capsid and capsid anchor. Viruses 6:1637-53 (2014). WB . PubMed: 24721788
- Lai MC et al. Human DDX3 interacts with the HIV-1 Tat protein to facilitate viral mRNA translation. PLoS One 8:e68665 (2013). IP . PubMed: 23840900
- Wright CA et al. Effects of Tat proteins and Tat mutants of different human immunodeficiency virus type 1 clades on glial JC virus early and late gene transcription. J Gen Virol 94:514-23 (2013). PubMed: 23152365
- Fitting S et al. Interactive Comorbidity between Opioid Drug Abuse and HIV-1 Tat: Chronic Exposure Augments Spine Loss and Sublethal Dendritic Pathology in Striatal Neurons. Am J Pathol 177:1397-410 (2010). IHC-Fr . PubMed: 20651230
- Henderson WW et al. Human immunodeficiency virus (HIV) type 1 Vpu induces the expression of CD40 in endothelial cells and regulates HIV-induced adhesion of B-lymphoma cells. J Virol 78:4408-20 (2004). PubMed: 15078922
- Kjerrström A et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). PubMed: 11352667
- Tähtinen M et al. B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother 51:480-7 (1997). PubMed: 9863510
- Ranki A et al. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS 9:1001-8 (1995). PubMed: 8527071
- Ranki A et al. Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef, Tat and Rev in acutely and chronically infected lymphoid cell lines. Arch Virol 139:365-78 (1994). PubMed: 7832642