Overview

  • Product name
    Anti-HIV1 tat antibody [1]
    See all HIV1 tat primary antibodies
  • Description
    Mouse monoclonal [1] to HIV1 tat
  • Host species
    Mouse
  • Specificity
    This antibody reacts with the rTat consensus sequences of HIV1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE).
  • Tested applications
    Suitable for: Radioimmunoprecipitation, IHC-P, IHC-Fr, ELISA, WBmore details
  • Immunogen

    Recombinant fragment corresponding to HIV-1 HIV1 tat.

  • Epitope
    Mapped to amino acids 2-9 (EPVDPRLE - B subtype consensus).
  • Positive control
  • General notes
    Also known as clone 02-001.

Properties

Applications

Our Abpromise guarantee covers the use of ab42359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Radioimmunoprecipitation Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20651230
ELISA 1/500 - 1/2000.
WB 1/500 - 1/2000.

Target

  • Relevance
    HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • Cellular localization
    Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
  • Alternative names
    • p14 antibody
    • Protein Tat antibody
    • Transactivating regulatory protein antibody

Images

  • Lane 1 : Anti-HIV1 tat antibody [1] (ab42359)


    Lane 1 : Recombinant HIV1 Tat (B subtype)
    Lane 2 : Protein size marker

References

This product has been referenced in:
  • Pereira LA  et al. MYB elongation is regulated by the nucleic acid binding of NF?B p50 to the intronic stem-loop region. PLoS One 10:e0122919 (2015). Read more (PubMed: 25853889) »
  • Vandergaast R  et al. Generation of West Nile virus infectious clones containing amino acid insertions between capsid and capsid anchor. Viruses 6:1637-53 (2014). WB . Read more (PubMed: 24721788) »
See all 10 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your responce. I have been unable to find data showing HIV1 tat in the cytoplasm. I am sorry that I could not be of more help.  I would recommend using positive and negative controls to ensure that your results are accurate. I would like to direct you to the HPA link which has some excellent images. http://www.proteinatlas.org/ENSG00000102241 Please let me know if you have any questions or if there is anything else Abcam can do to help you meet your research goals.

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Answer

Thank you for contacting Abcam.

I am sorry to hear of this issue with our product, especially after your earlier work seemed to have exciting implications. This product is very well mapped and I am surprised to hear of the results that you have seen you have tested in multiple negative (uninfected) control tissues in IHC-P.

Would you be able to send me detailed information about your protocol? It would also be very helpful to see images of your stains. With this information I may be able to offer suggestions that may help or to offer a replacement or refund if we cannot resolve these issues.

I look forward to your reply. Please let me know if you have any other questions.

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Answer

Thank you for your reply. The information that you have sent and the tissue that you are staining on would seem to explain the membrane staining nicely. I certainly do appreciate your finding this for me and will add this information to our notes.  If you perform any staining on test samples of HIV infected but treated patients would you please let me know the results? Thanks again. If there is anything else that we can do for you, please do not hesitate to contact us.

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Answer

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I would like to gather further information regarding the samples tested and the protocol used to see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. Could you please send me your protocol, including but not limited to:   Tissue preparation and fixation procedures. Any permeablization or antigen retrieval steps that you use. Blocking solution compositon, blocking time and temperature. Concentrations of the antibodies that you have tried, how long they were incubated and at what temperature. Any positive control tissues that you have used and what the results where of those. Any negative controls that you performed. Any images that you may have will be of great help as well. Thank you in advance for collecting this information for me.  If you have any questions please feel free to contact me. I look forward to receiving your reply.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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