Overview

  • Product name
  • Description
    Chicken polyclonal to HIV1 tat
  • Host species
    Chicken
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    This antibody may react to various subtypes of HIV 1 tat protein.
  • Immunogen

    Full length HIV 1 tat protein (Human).

  • Positive control
  • General notes
    During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab14029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 12 kDa.

Target

  • Relevance
    HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • Cellular localization
    Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
  • Alternative names
    • p14 antibody
    • Protein Tat antibody
    • Transactivating regulatory protein antibody

References

ab14029 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for your enquiry. Below please find the protocol used for blotting with this antibody. The antibody dilution was 1:2000, and recombinant fusion protein used as the antigen. I hope this information helps. Please do not hesitate to contact us if you need anything further. Protocol for Western Blot Performed with IgY Materials 1. Tris-buffered Saline-Tween 20 solution (TBS-T): 1.21 g Tris-base (10 mM), 8.77 g NaCl (150 mM), in 1 L of H20, pH 7.4, containing 0.05% (v/v) Tween-20. 2. Non-fat dry milk. 3. HRP-conjugated anti-IgY secondary. 4. Immun-blot colorimetric assay kit (Bio-Rad Catalog #: 170-8235). Method 1. Separate appropriate amount of cell lysates (1-10 ul of 0.5 mg/ml each lane) by 10-20% SDS-PAGE, followed by transferring to PVDF membrane. 2. Block membrane with 5% non-fat milk in TBS-T (Tris-buffered saline containing 0.05% Tween, pH 7.4) for 1 hour at room temperature or longer at 4C. (BSA is not recommended as a blocking reagent). 3. Rinse membrane with TBS-T. 4. Incubate membrane with IgY antibodies appropriately diluted with 5% milk in TBS-T @ R.T. for 1 h. 5. Wash membrane with TBS-T, 3 min each, total of 3 times. 6. Incubate with 2nd antibody @ R.T. for 1 h. 7. Wash with TBS-T, 3-5 min each with shaking, total of 3 times. 8. Perform color development. Or perform ECL detection of signal using Pierce ECL kit.

Read More

Answer

I'm sorry to hear you are having a problem with ab14029, thank you for taking the time to fill in the questionnaire, it is very useful. I would like to suggest the following modifications to your protocol: -fix your cells for 5-10min in ice cold acetone or methanol (a longer fixation may be responsible for the non specific binding) -block one hour in 5% BSA (you may wish to try your blocking solution but we tend to recommend 5%BSA in PBST or PBS) -incubate your primary antibody overnight in PBS (or PBST) overnight at 1:200, try a lower dilution (maybe 1:100). Please let me know if this helps and do not hesitate to contact us if you still have problems,

Read More

Answer

We don't know the viral strain and the source of virus. However, this antibody should recognize tat from various stains since this is a polyclonal antibody against the full length protein.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up