Biotin Anti-HIV1 tat antibody (ab43015)
Key features and details
- Biotin Rabbit polyclonal to HIV1 tat
- Suitable for: WB, ELISA, Flow Cyt
- Reacts with: Human immunodeficiency virus
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-HIV1 tat antibody
See all HIV1 tat primary antibodies -
Description
Biotin Rabbit polyclonal to HIV1 tat -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Recombinant full length protein expressed in E. coli
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 0.05% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab43015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
ELISA | ||
Flow Cyt |
Notes |
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Flow Cyt: 1/200. Use Streptavidin-FITC conjugated detection reagent.
WB: 1/1000. Predicted molecular weight: 14 kDa. Use Streptavidin (HRP) conjugated detection reagent.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR. -
Cellular localization
Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region. -
Database links
- Entrez Gene: 155871 Human immunodeficiency virus
- SwissProt: P04608 Human immunodeficiency virus
- SwissProt: P04610 Human immunodeficiency virus
- SwissProt: Q6UFJ9 Human immunodeficiency virus
- SwissProt: Q900K4 Human immunodeficiency virus
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Alternative names
- p14 antibody
- Protein Tat antibody
- Transactivating regulatory protein antibody
Datasheets and documents
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Datasheet download
References (3)
ab43015 has been referenced in 3 publications.
- Swamy G et al. IL-1Ra deficiency accelerates intervertebral disc degeneration in C57BL6J mice. JOR Spine 5:e1201 (2022). PubMed: 35783913
- Dickens AM et al. Chronic low-level expression of HIV-1 Tat promotes a neurodegenerative phenotype with aging. Sci Rep 7:7748 (2017). ELISA . PubMed: 28798382
- Johnson TP et al. Induction of IL-17 and nonclassical T-cell activation by HIV-Tat protein. Proc Natl Acad Sci U S A 110:13588-93 (2013). PubMed: 23898208