Anti-HIV1 tat antibody - ChIP Grade (ab43014)
Key features and details
- Rabbit polyclonal to HIV1 tat
- Suitable for: WB, EMSA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
Overview
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Product name
Anti-HIV1 tat antibody - ChIP Grade
See all HIV1 tat primary antibodies -
Description
Rabbit polyclonal to HIV1 tat -
Host species
Rabbit -
Tested applications
Suitable for: WB, EMSAmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Recombinant full length protein expressed in E.coli.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab43014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
Use at an assay dependent concentration.
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EMSA |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
EMSA
Use at an assay dependent concentration. |
Target
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Relevance
HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR. -
Cellular localization
Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region. -
Database links
- Entrez Gene: 155871 Human immunodeficiency virus
- SwissProt: P04608 Human immunodeficiency virus
- SwissProt: P04610 Human immunodeficiency virus
- SwissProt: Q6UFJ9 Human immunodeficiency virus
- SwissProt: Q900K4 Human immunodeficiency virus
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Alternative names
- p14 antibody
- Protein Tat antibody
- Transactivating regulatory protein antibody
Images
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All lanes : Anti-HIV1 tat antibody - ChIP Grade (ab43014) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate 0 hours after infection
Lane 2 : Jurkat whole cell lysate 2 hours after infection
Lane 3 : Jurkat whole cell lysate 4 hours after infection
Lane 4 : Jurkat whole cell lysate 6 hours after infection
Lane 5 : Jurkat whole cell lysate 8 hours after infection
Lane 6 : Jurkat whole cell lysate 10 hours after infection
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 14 kDa why is the actual band size different from the predicted?
Jurkat cells were pNL4.3 infected and samples taken at 2 hour intervals for blotting.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab43014 has been referenced in 23 publications.
- Ait-Ammar A et al. Inhibition of HIV-1 gene transcription by KAP1 in myeloid lineage. Sci Rep 11:2692 (2021). PubMed: 33514850
- Chandra PK et al. Latent HIV-Exosomes Induce Mitochondrial Hyperfusion Due to Loss of Phosphorylated Dynamin-Related Protein 1 in Brain Endothelium. Mol Neurobiol 58:2974-2989 (2021). PubMed: 33586027
- Alves de Souza Rios L et al. HIV-1 Transactivator of Transcription (Tat) Co-operates With AP-1 Factors to Enhance c-MYC Transcription. Front Cell Dev Biol 9:693706 (2021). PubMed: 34277639
- Chakraborty S et al. A Stronger Transcription Regulatory Circuit of HIV-1C Drives the Rapid Establishment of Latency with Implications for the Direct Involvement of Tat. J Virol 94:N/A (2020). PubMed: 32669338
- Joshi CR et al. Astrocyte HIV-1 Tat Differentially Modulates Behavior and Brain MMP/TIMP Balance During Short and Prolonged Induction in Transgenic Mice. Front Neurol 11:593188 (2020). PubMed: 33384653