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I have recently purchased Rb pAb to HIV1 tat (cat#: ab43014 ) from you twice. The antibody coming with the first order worked very well for both Western Blot and ChIP experiments, so we decided to buy more. We ordered two new vials of this antibody, unfortunately they seem not working for neither Western Blot nor ChIP experiments this time. The lot number for the tat antibody is GR87684-1. I wonder if you have validated new lots of this tat antibody and if it is possible that you could replace the two vials of antibody of our second order.
Asked on Aug 20 2012
Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
What is the problem that you are experiencing? Is it no bands in WB or enrichment in ChIP? I am listing some questions below so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
1) Abcam product code ab43014
2) Abcam order reference number or product batch number GR87684-1
3) Description of the problem
4) Sample preparation:
Type of sample (whole cell lysates, fraction, recombinant protein…):
Boiling for ≥5 min? yes/no
Protein loaded ug/lane or cells/lane
5) Percentage of gel
Type of membrane
Protein transfer verified
Blocking agent and concentration
6) Primary antibody (If more than one was used, describe in “additional notes”) :
Concentration or dilution
7) Secondary antibody:
Concentration or dilution
Fluorochrome or enzyme conjugate:
8) Washing after primary and secondary antibodies:
Number of washes
10) How many times have you run this staining?
Do you obtain the same results every time?
What steps have you altered to try and optimize the use of this antibody?
Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.
I look forward to receiving your reply.
Answered on Aug 20 2012