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Thanks for helping us investigate this case. I am sorry for the late response.
As I mentioned in my previous email, the Tat antibody of our first order worked very well for both Western Blot and ChIP experiments. However the antibodies of the second order didn’t work. There is no bands in WB even after long exposure and no specific signals for ChIP. Please find the attached word file for the answers of your questions. Since there are no bands in WB, I didn’t scan the films. But I attached a WB result with the antibody (1:1000 dilution) came in the first order, which showed clear band after 30 second exposure.
Asked on Sep 05 2012
Thank you for your reply and for providing the additional information.
It appears that the antibody isn't working as expected, so I would like to offer you a free of charge replacement or credit note. We only have the same lot in stock in our US office as the one you tried that didn't work well. We do have 1 vial of the old lot that worked well GR65428-5, but this would ship from our UK office and will take a few extra days.
How would you like to proceed? Would you like us to send you a replacement vial from the old lot that worked well?
Answered on Sep 05 2012