Anti-HIV1 tat antibody (FITC) (ab43016)


  • Product name
    Anti-HIV1 tat antibody (FITC)
    See all HIV1 tat primary antibodies
  • Description
    Rabbit polyclonal to HIV1 tat (FITC)
  • Host species
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody is specific for Tat protein and GST-Tat fusion protein.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    Recombinant full length protein expressed in E. coli.



Our Abpromise guarantee covers the use of ab43016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: 1/200.
    For general use, dilute at 1:1 (V/V) in PBS.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
    • Cellular localization
      Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
    • Alternative names
      • p14 antibody
      • Protein Tat antibody
      • Transactivating regulatory protein antibody


    • ab43016 at a 1/200 dilution.


    ab43016 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Please find attached to this email theTAT sequence used for the epitope mapping. "TAT 1-20" corresponds to amino acids 1 to 20.

    Concerning the cell localisation, we recommend to permeabilise the cells with a standard protocol using Trito...

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    Please find attached to this email the TAT sequence from clade B used for the epitope mapping, with reactivity scoring for different peptides. "TAT 1-20" corresponds to amino acids 1 to 20. Your nucleotide substitution is within that range and we do no...

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    Thank you for getting back in contact and letting me know of your progress.

    I am sorry to hear that ab63957 has as yet not produced the desired results using flow cytometry. I hope that using the new secondary antibody may provide you with be...

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    Merci pour votre réponse.

    Je me permets de vous transférer le numéro de commande de remplacement gratuit de ce produit : xx . Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

    J'espère que vo...

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    Merci de nous avoir contactés. Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu.

    Nous allons vous remplacer ce produit avec un nouveau tube. Par contre j'aimerai bien attirer votre attention s...

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    Thank you for your inquiry and your patience.

    Indeed, the questionsyou raised are very important. I have contacted the laboratory in order to find out more about this antibody.
    The antibody against Tat has been generated against the full...

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    I'm sorry I was not available to deal with your query yesterday. I have generated the 50% discount for ab63957 as discussed which will be sent in a separate email. Let me know if you do not receive it. The testing discount code for this antibody is...

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    Thank you for letting me know of your progress. I am still waiting for a response as to the exact epitope recognised by this antibody, it may be as we discussed over the phone that the antibody is not able to recognise the portion of Tat-protein wh...

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