Key features and details
- Mouse monoclonal [N3] to HIV1 tat
- Suitable for: ELISA, WB
- Isotype: IgM
Product nameAnti-HIV1 tat antibody [N3]
See all HIV1 tat primary antibodies
DescriptionMouse monoclonal [N3] to HIV1 tat
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Other species
Recombinant full length protein corresponding to HIV1 tat. (Recombinant Tat protein of HIV-1 HXB2, purified from E. coli)
EpitopeMapped to amino acids 47-58 (YGRKKRRQRRRA – B subtype a.a. consensus, Tat basic domain, Swissprot ID: P04608)
General notesAlso known as clone 02-010.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab63957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 1 kDa.|
RelevanceHIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
Cellular localizationNuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
- p14 antibody
- Protein Tat antibody
- Transactivating regulatory protein antibody
ab63957 has been referenced in 11 publications.
- Dong H et al. Role of FOXO3 Activated by HIV-1 Tat in HIV-Associated Neurocognitive Disorder Neuronal Apoptosis. Front Neurosci 13:44 (2019). PubMed: 30778283
- Parker AR et al. Modulator-Gated, SUMOylation-Mediated, Activity-Dependent Regulation of Ionic Current Densities Contributes to Short-Term Activity Homeostasis. J Neurosci 39:596-611 (2019). PubMed: 30504282
- Zhang Y et al. nNOS-CAPON interaction mediates amyloid-ß-induced neurotoxicity, especially in the early stages. Aging Cell 17:e12754 (2018). PubMed: 29577585
- Ye X et al. HIV-1 Tat inhibits EAAT-2 through AEG-1 upregulation in models of HIV-associated neurocognitive disorder. Oncotarget 8:39922-39934 (2017). PubMed: 28404980
- Wang J et al. Menin mediates Tat-induced neuronal apoptosis in brain frontal cortex of SIV-infected macaques and in Tat-treated cells. Oncotarget 8:18082-18094 (2017). IF ; Rhesus monkey . PubMed: 28178646