Overview

  • Product name

    Anti-HIV1 tat antibody [N3]
    See all HIV1 tat primary antibodies
  • Description

    Mouse monoclonal [N3] to HIV1 tat
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC, ELISA, WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant full length protein corresponding to HIV1 tat. (Recombinant Tat protein of HIV-1 HXB2, purified from E. coli)

  • Epitope

    Mapped to amino acids 47-58 (YGRKKRRQRRRA – B subtype a.a. consensus, Tat basic domain, Swissprot ID: P04608)
  • Positive control

  • General notes

    Also known as clone 02-010.

Properties

Applications

Our Abpromise guarantee covers the use of ab63957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration. PubMed: 25653431
ELISA Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 1 kDa.

Target

  • Relevance

    HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • Cellular localization

    Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
  • Alternative names

    • p14 antibody
    • Protein Tat antibody
    • Transactivating regulatory protein antibody

Images

  • IHC-P image of HIV1 tat staining on Transgenic mouse olfactory bulb. Heat mediated antigen retrieval was performed using trisodium citrate (pH 6). The tissue section was permeabilization using Triton x100. To block non-specififc binding, sections were blocked in 2% BSA in PBS.

    See Abreview

  • Anti-HIV1 tat antibody [N3] (ab63957) + Recombinant HIV1 Tat

References

This product has been referenced in:

  • Dong H  et al. Role of FOXO3 Activated by HIV-1 Tat in HIV-Associated Neurocognitive Disorder Neuronal Apoptosis. Front Neurosci 13:44 (2019). Read more (PubMed: 30778283) »
  • Parker AR  et al. Modulator-Gated, SUMOylation-Mediated, Activity-Dependent Regulation of Ionic Current Densities Contributes to Short-Term Activity Homeostasis. J Neurosci 39:596-611 (2019). Read more (PubMed: 30504282) »
See all 11 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. However, in this case we wouldn't be able to guarantee the antibody will work to bind your peptide bound to an enzyme.

I would probably recommend the polyclonal rather than the monoclonal since the polyclonal will have multiple binding epitopes. However, ab6539 hasn't been tested in immunohistochemistry. If you would like to test this antibody in IHC, let me know as you would qualify for our testing discount program. Essentially you would purchase this antibody, test it in IHC, submit an Abreview with your data (whether positive or negative) and receive a discount code for a free antibody off your next Abcam purchase within 4 months.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab6539.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Western blot
Sample
HIV Recombinant protein (recombinant bacterial protein with TAT-domain and)
Loading amount
4 µg
Specification
recombinant bacterial protein with TAT-domain and
Treatment
IPTG
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Stefan Krautwald

Verified customer

Submitted Mar 09 2012

Question
Answer

Thank you for contacting us and reporting the problems encountered with using Anti-HIV1 tat antibody [N3] (ab63957).

We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As discussed over the phone Ihave attached a questionnaire so thatI can gather further information regarding the samples tested and the protocol used. If you could include any images of the gels you have run using this antibody that would be very helpful. OnceI have receive the completed questionnaire,I will look at the protocol and see if there are any suggestionsI can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: trisodium citrate at pH 6.0
Permeabilization
Yes - Tritonx100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 07 2010

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