Overview

  • Product name

    Anti-HIV1 tat antibody [N3]
    See all HIV1 tat primary antibodies
  • Description

    Mouse monoclonal [N3] to HIV1 tat
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC, ELISA, WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant full length protein corresponding to HIV1 tat. (Recombinant Tat protein of HIV-1 HXB2, purified from E. coli)

  • Epitope

    Mapped to amino acids 47-58 (YGRKKRRQRRRA – B subtype a.a. consensus, Tat basic domain, Swissprot ID: P04608)
  • Positive control

  • General notes

    Also known as clone 02-010.

Properties

Applications

Our Abpromise guarantee covers the use of ab63957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration. PubMed: 25653431
ELISA Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 1 kDa.

Target

  • Relevance

    HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • Cellular localization

    Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
  • Alternative names

    • p14 antibody
    • Protein Tat antibody
    • Transactivating regulatory protein antibody

Images

  • IHC-P image of HIV1 tat staining on Transgenic mouse olfactory bulb. Heat mediated antigen retrieval was performed using trisodium citrate (pH 6). The tissue section was permeabilization using Triton x100. To block non-specififc binding, sections were blocked in 2% BSA in PBS.

    See Abreview

  • Anti-HIV1 tat antibody [N3] (ab63957) + Recombinant HIV1 Tat

References

This product has been referenced in:

  • Dong H  et al. Role of FOXO3 Activated by HIV-1 Tat in HIV-Associated Neurocognitive Disorder Neuronal Apoptosis. Front Neurosci 13:44 (2019). Read more (PubMed: 30778283) »
  • Zhang Y  et al. nNOS-CAPON interaction mediates amyloid-ß-induced neurotoxicity, especially in the early stages. Aging Cell 17:e12754 (2018). Read more (PubMed: 29577585) »
See all 10 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Western blot
Sample
HIV Recombinant protein (recombinant bacterial protein with TAT-domain and)
Loading amount
4 µg
Specification
recombinant bacterial protein with TAT-domain and
Treatment
IPTG
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Stefan Krautwald

Verified customer

Submitted Mar 09 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: trisodium citrate at pH 6.0
Permeabilization
Yes - Tritonx100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 07 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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