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Inquiry: We are using a fusion protein of the TAT (Transactivator of Transcription) peptide of the HIV virus to allow our enzime to enter in the cells. Using enzimatic activity, we were able to see that the TAT fusion protein is working in vitro, but we wanted to try it in vivo and we have some extra problems: endogenous activity of our enzime and higher variability between samples. We would like to have a specific antibody to recognize the TAT peptide by western blot and, if possible, by immuno. Looking in your web page I found a couple of possible candidates ab63957 and ab6539, but some things are not clear: 1/ Will these antibodies (or any others you can recommend) recognize the TAT peptide attached to our enzime? 2/ If so, would it be possible to get a sample to try it first in our system? It would be great if the antibody was in mouse or rabbit instead of sheep.. Thank you very much.
Asked on Jun 05 2012
Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. However, in this case we wouldn't be able to guarantee the antibody will work to bind your peptide bound to an enzyme.
I would probably recommend the polyclonal rather than the monoclonal since the polyclonal will have multiple binding epitopes. However, ab6539 hasn't been tested in immunohistochemistry. If you would like to test this antibody in IHC, let me know as you would qualify for our testing discount program. Essentially you would purchase this antibody, test it in IHC, submit an Abreview with your data (whether positive or negative) and receive a discount code for a free antibody off your next Abcam purchase within 4 months.
To find out more about our Abreview system, please see the following link:
I hope this information is helpful. Please do not hesitate to contact us again with any other questions.
Answered on Jun 05 2012