Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HIV1 tat antibody [N3]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: trisodium citrate at pH 6.0
Permeabilization
Yes - Tritonx100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C

Other product details

Dilution
1/200
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Dako Goat Anti Mouse
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Dilution
1/100

Additional data

Additional Notes
Transgenic mouse HIV tat promoter. Olfactory bulb stained very beutifully with DAB.

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 07 2010

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