Western blot abreview for Anti-HIV1 tat antibody [N3]

Poor
Abreviews
Application
Western blot
Sample
HIV Recombinant protein (recombinant bacterial protein with TAT-domain and)
Loading amount
4 µg
Specification
recombinant bacterial protein with TAT-domain and
Treatment
IPTG
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 5% BSA

Secondary antibody

Name
Non-Abcam antibody was used: mouse-IgG-HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 80, 70, 30, 25 kDa
Positive control
specific detection with anti-His-Tag antibody

Additional data

Additional Notes
?identical amounts of the following probes were loaded in A (commassie stained) and B (WB):

01 = 4 µg whole cell lysate before IPTG stimulation
02 = 4 µg whole cell lysate after IPTG stimulation
03 = 4 µg soluble fraction before centrifugation
04 = aliquot of no 3 after centrifugation
05 = 4 µg soluble fraction before chromatography
06 = aliquot of wash fraction (wash solution cont. 10 mM imidazole)
07 = aliquot of wash fraction (wash solution cont. 15 mM imidazole)
08 = 2 µg eluate before concentration
09 = 2 µg eluate after concentration
10 = PP marker
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

Dr. Stefan Krautwald

Verified customer

Submitted Mar 09 2012

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