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  1. Link

    hiv2-nef-antibody-4b5-ab22626.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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Anti-HIV2 Nef antibody [4B5] (ab22626)

  • Datasheet
  • SDS
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Key features and details

  • Rat monoclonal [4B5] to HIV2 Nef
  • Suitable for: WB, IP
  • Isotype: IgG1

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Overview

  • Product name

    Anti-HIV2 Nef antibody [4B5]
  • Description

    Rat monoclonal [4B5] to HIV2 Nef
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Fusion protein: TrpE-Fusion protein

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    4B5
  • Myeloma

    x63-Ag8.653
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab22626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.
IP
Use at an assay dependent dilution.
Notes
WB
Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.
IP
Use at an assay dependent dilution.

Target

  • Relevance

    Nef is a multifunctional protein of 27 kDa in HIV1 and 35 kDa in HIV2. It induces the endocytosis of CD4 Receptor and reduces the MHC1 Receptors. Thereby it enhances viral infectivity and affects T-cell signalling.
  • Cellular localization

    Cell Membrane
  • Alternative names

    • F-protein antibody
    • HIV2 nef protein antibody
    • Human immunodeficiency virus 2 nef antibody
    • Nef protein antibody
    • Negative factor antibody
    see all

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab22626? Please let us know so that we can cite the reference in this datasheet.

ab22626 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Western blot abreview for Anti-HIV2 Nef antibody [4B5]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
transfected with HIV2Nef-GFP
Specification
HEK 293T
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Oct 07 2020

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