Key features and details
- Mouse monoclonal [2417C2 a] to HIVEP1
- Suitable for: WB, Dot blot
- Reacts with: Recombinant fragment
- Isotype: IgG1
Product nameAnti-HIVEP1 antibody [2417C2 a]
DescriptionMouse monoclonal [2417C2 a] to HIVEP1
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Human
Recombinant C terminal fragment of human HIVEP1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesab58652 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Clone number2417C2 a
Our Abpromise guarantee covers the use of ab58652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 297 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionThis protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV-1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, and interferon-beta genes. It may act in T-cell activation. Involved in activating HIV-1 gene expression. Isoform 2 and isoform 3 also bind to the IPCS (IRF1 and p53 common sequence) DNA sequence in the promoter region of interferon regulatory factor 1 and p53 genes and are involved in transcription regulation of these genes. Isoform 2 does not activate HIV-1 gene expression. Isoform 2 and isoform 3 may be involved in apoptosis.
Sequence similaritiesContains 5 C2H2-type zinc fingers.
DomainContains two sets of 2 zinc-fingers, which are widely separated and recognize the same DNA sequence. There is a fifth zinc-finger in-between.
Cellular localizationCytoplasm. Nucleus and Nucleus.
- Information by UniProt
- Cirhin interaction protein antibody
- CIRIP antibody
- CRYBP1 antibody
Anti-HIVEP1 antibody [2417C2 a] (ab58652) + immunising recombinant protein fragment
Predicted band size: 297 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab58652 has not yet been referenced specifically in any publications.