Overview

  • Product name

    Anti-HIVEP2 antibody [2418C2a]
  • Description

    Mouse monoclonal [2418C2a] to HIVEP2
  • Host species

    Mouse
  • Tested applications

    Suitable for: Dot blot, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment of human HIVEP2 from near the C terminus.

  • Positive control

    • Recombinant human HIVEP2.

Properties

Applications

Our Abpromise guarantee covers the use of ab70599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
WB
  • Application notes
    Dot: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects bands of approximately 35 and 39 kDa (predicted molecular weight: 269 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, somatostatin receptor II, and interferon-beta genes. It may act in T-cell activation.
    • Tissue specificity

      Expressed in brain and skeletal muscle.
    • Sequence similarities

      Contains 4 C2H2-type zinc fingers.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • HIV EP2 antibody
      • HIV-EP2 antibody
      • HIVEP2 antibody
      • human immunodeficiency virus type I enhancer binding protein 2 antibody
      • Human immunodeficiency virus type I enhancer-binding protein 2 antibody
      • MBP 2 antibody
      • MBP-2 antibody
      • MHC binding protein 2 antibody
      • MHC-binding protein 2 antibody
      • MIBP1 antibody
      • SHN2 antibody
      • Transcription factor HIVEP2 antibody
      • ZAS2 antibody
      • ZEP2_HUMAN antibody
      • ZNF40B antibody
      see all

    Images

    • Anti-HIVEP2 antibody [2418C2a] (ab70599) + immunising recombinant protein

      Predicted band size: 269 kDa
      Observed band size: 35,39 kDa
      why is the actual band size different from the predicted?



      The molecular weight of the bands on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

    References

    This product has been referenced in:

    • Bash-Imam Z  et al. Translational reprogramming of colorectal cancer cells induced by 5-fluorouracil through a miRNA-dependent mechanism. Oncotarget 8:46219-46233 (2017). Read more (PubMed: 28515355) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Question
    Answer

    DISCOUNT CODE: *******
    Expiration date: ***********

    I am very pleased to hear you would like to accept our offer and test ab70599 in rat tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forrat tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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