Key features and details
- Mouse monoclonal [2418C2a] to HIVEP2
- Suitable for: Dot blot, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-HIVEP2 antibody [2418C2a]
DescriptionMouse monoclonal [2418C2a] to HIVEP2
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Human
Recombinant fragment of human HIVEP2 from near the C terminus.
- Recombinant human HIVEP2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate
Concentration information loading...
PurityProtein G purified
Purification notesab70599 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab70599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects bands of approximately 35 and 39 kDa (predicted molecular weight: 269 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThis protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, somatostatin receptor II, and interferon-beta genes. It may act in T-cell activation.
Tissue specificityExpressed in brain and skeletal muscle.
Sequence similaritiesContains 4 C2H2-type zinc fingers.
- Information by UniProt
- HIV EP2 antibody
- HIV-EP2 antibody
- HIVEP2 antibody
Anti-HIVEP2 antibody [2418C2a] (ab70599) + immunising recombinant protein
Predicted band size: 269 kDa
Observed band size: 35,39 kDa why is the actual band size different from the predicted?
The molecular weight of the bands on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab70599 has been referenced in 1 publication.
- Bash-Imam Z et al. Translational reprogramming of colorectal cancer cells induced by 5-fluorouracil through a miRNA-dependent mechanism. Oncotarget 8:46219-46233 (2017). PubMed: 28515355