Anti-HJURP antibody (ab100800)
Key features and details
- Rabbit polyclonal to HJURP
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-HJURP antibody
See all HJURP primary antibodies -
Description
Rabbit polyclonal to HJURP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HJURP aa 650-750. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8NCD3 -
General notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified using an epitope specific to HJURP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 µg/ml. Predicted molecular weight: 84 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/5000.
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Notes |
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WB
Use a concentration of 0.04 µg/ml. Predicted molecular weight: 84 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/5000. |
Target
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Relevance
Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions. -
Cellular localization
Nucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage. -
Database links
- Entrez Gene: 55355 Human
- Omim: 612667 Human
- SwissProt: Q8NCD3 Human
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Alternative names
- 14-3-3-associated AKT substrate antibody
- FAKTS antibody
- fetal liver expressing gene 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling HJURP with ab100800 at 1/5000 (0.2µg/mg). Detection: DAB.
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All lanes : Anti-HJURP antibody (ab100800) at 0.04 µg/ml
Lane 1 : K-562 whole cell lysate
Lane 2 : U2OS whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF-7 whole cell lysate
Lysates/proteins at 2 µg per lane.
Predicted band size: 84 kDa -
ab100800 at 1 µg/ml staining HJURP in HeLa cell lysate immunoprecipitated using ab100800 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab100800 has been referenced in 7 publications.
- Yang Y et al. The expression, clinical relevance, and prognostic significance of HJURP in cholangiocarcinoma. Front Oncol 12:972550 (2022). PubMed: 35965590
- Lai W et al. HJURP promotes proliferation in prostate cancer cells through increasing CDKN1A degradation via the GSK3ß/JNK signaling pathway. Cell Death Dis 12:583 (2021). PubMed: 34099634
- Yuan JS et al. Holliday junction-recognition protein modulates apoptosis, cell cycle arrest and reactive oxygen species stress in human renal cell carcinoma. Oncol Rep 44:1246-1254 (2020). PubMed: 32582972
- Pan D et al. Mechanism of centromere recruitment of the CENP-A chaperone HJURP and its implications for centromere licensing. Nat Commun 10:4046 (2019). PubMed: 31492860
- Liu C & Mao Y Diaphanous formin mDia2 regulates CENP-A levels at centromeres. J Cell Biol 213:415-24 (2016). PubMed: 27185834
- Montes de Oca R et al. The histone chaperone HJURP is a new independent prognostic marker for luminal A breast carcinoma. Mol Oncol 9:657-74 (2015). PubMed: 25497280
- Bravaccini S et al. New biomarkers to predict the evolution of in situ breast cancers. Biomed Res Int 2014:159765 (2014). IHC ; Human . PubMed: 25243117