Key features and details
- Rabbit polyclonal to HJURP
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HJURP antibody
See all HJURP primary antibodies
DescriptionRabbit polyclonal to HJURP
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- ICC/IF: A431 cells. IHC-P: Human lymph node, testis, skin and stomach tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceCentromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
Cellular localizationNucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for HJURP (green) using ab224076 (4 µg/ml) in ICC/IF.
Paraffin embedded human stomach tissue stained for HJURP with ab224076 (1/200 dilution) in immunohistochemical analysis.
Paraffin embedded human skin tissue stained for HJURP with ab224076 (1/200 dilution) in immunohistochemical analysis.
Paraffin embedded human testis tissue stained for HJURP with ab224076 (1/200 dilution) in immunohistochemical analysis.
Paraffin embedded human lymph node tissue stained for HJURP with ab224076 (1/200 dilution) in immunohistochemical analysis.
ab224076 has been referenced in 1 publication.
- Xiang Y et al. In vitro expansion affects the response of human bone marrow stromal cells to irradiation. Stem Cell Res Ther 10:82 (2019). PubMed: 30850008