Overview

  • Product name

    Anti-HJURP antibody [EPR22619-41]
    See all HJURP primary antibodies
  • Description

    Rabbit monoclonal [EPR22619-41] to HJURP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human HJURP aa 1-200. The exact sequence is proprietary.
    Database link: Q8NCD3

  • Positive control

    • WB: HeLa and HEK-293 whole cell lysates; HeLa nuclear fraction; human tonsil lysate. IHC-P: Human thymus and tonsil tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22619-41
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Relevance

      Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
    • Cellular localization

      Nucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.
    • Database links

    • Alternative names

      • 14-3-3-associated AKT substrate antibody
      • FAKTS antibody
      • fetal liver expressing gene 1 antibody
      • Fetal liver-expressing gene 1 protein antibody
      • FLEG1 antibody
      • hFLEG1 antibody
      • HJURP antibody
      • Holliday junction recognition protein antibody
      • Up-regulated in lung cancer 9 antibody
      • URLC9 antibody
      see all

    Images

    • Anti-HJURP antibody [EPR22619-41] (ab233541) at 1/1000 dilution + HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 84 kDa
      Observed band size: 84 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 23771058).

    • All lanes : Anti-HJURP antibody [EPR22619-41] (ab233541) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HeLa nuclear fraction
      Lane 3 : HeLa cytoplasmic fraction
      Lane 4 : Human tonsil tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 84 kDa
      Observed band size: 84 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times.

      Lanes 1-3: 48 seconds; Lane 4: 3.25 seconds.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 23771058).

      Lanes 1-3 in this blot were developed using a higher sensitivity ECL substrate.

    • Immunohistochemical analysis of paraffin-embeddded human tonsil tissue labeling HJURP with ab233541 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human tonsil germinal center is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      The section was incubated with ab233541 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

       

    • Immunohistochemical analysis of paraffin-embeddded human thymus tissue labeling HJURP with ab233541 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on thymocytes of human thymus cortex (PMID: 17256767) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      The section was incubated with ab233541 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

       

    References

    ab233541 has not yet been referenced specifically in any publications.

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