Overview

  • Product name

    Anti-HJURP antibody [HOPE169E]
    See all HJURP primary antibodies
  • Description

    Mouse monoclonal [HOPE169E] to HJURP
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein (proprietary-tag) corresponding to Human HJURP aa 1-748.
    Database link: Q8NCD3

  • Positive control

    • K562, Daudi and Raji cell lysates; Human kidney lysate; HEK293T cells transfected with HJURP; Human tonsil, tonsil epithelium, thymus and testicle tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    HOPE169E
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Predicted molecular weight: 84 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/200.

Target

  • Relevance

    Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
  • Cellular localization

    Nucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.
  • Database links

  • Alternative names

    • 14-3-3-associated AKT substrate antibody
    • FAKTS antibody
    • fetal liver expressing gene 1 antibody
    • Fetal liver-expressing gene 1 protein antibody
    • FLEG1 antibody
    • hFLEG1 antibody
    • HJURP antibody
    • Holliday junction recognition protein antibody
    • Up-regulated in lung cancer 9 antibody
    • URLC9 antibody
    see all

Images

  • Immunohistochemical analysis of frozen cytospin preparations of HEK293T cells expressing HJURP (left and right panel) or A-MYB (central panel) as negative control. The left and central panels were incubated with ab175577, labeling HJURP. The right panel was incubated with an antibody against the expression vector itself (positive control).

  • All lanes : Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470) at 1/100 dilution

    Lane 1 : Human kidney lysate
    Lane 2 : Human colon lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Daudi cell lysate
    Lane 5 : Raji cell lysate

    Lysates/proteins at 100 µg per lane.

    Predicted band size: 84 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil, tonsil epithelium, thymus and testicle tissues, labeling HJURP using ab175577 at a 1/400 dilution.

  • Immunofluorescence analysis of Human tonsil tissue, labeling HJURP using ab175577 at a 1/200 dilution (green), followed by washing, antifading and DAPI staining (blue).

References

This product has been referenced in:

  • Li L  et al. Increased Expression of Holliday Junction-Recognizing Protein (HJURP) as an Independent Prognostic Biomarker in Advanced-Stage Serous Ovarian Carcinoma. Med Sci Monit 24:3050-3055 (2018). Read more (PubMed: 29743473) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175577.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up