Product nameAnti-HJURP antibody [HOPE169E]
See all HJURP primary antibodies
DescriptionMouse monoclonal [HOPE169E] to HJURP
Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein (proprietary-tag) corresponding to Human HJURP aa 1-748.
Database link: Q8NCD3
- K562, Daudi and Raji cell lysates; Human kidney lysate; HEK293T cells transfected with HJURP; Human tonsil, tonsil epithelium, thymus and testicle tissues.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab175577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Predicted molecular weight: 84 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceCentromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
Cellular localizationNucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.
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Immunohistochemical analysis of frozen cytospin preparations of HEK293T cells expressing HJURP (left and right panel) or A-MYB (central panel) as negative control. The left and central panels were incubated with ab175577, labeling HJURP. The right panel was incubated with an antibody against the expression vector itself (positive control).
All lanes : Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470) at 1/100 dilution
Lane 1 : Human kidney lysate
Lane 2 : Human colon lysate
Lane 3 : K562 cell lysate
Lane 4 : Daudi cell lysate
Lane 5 : Raji cell lysate
Lysates/proteins at 100 µg per lane.
Predicted band size: 84 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil, tonsil epithelium, thymus and testicle tissues, labeling HJURP using ab175577 at a 1/400 dilution.
Immunofluorescence analysis of Human tonsil tissue, labeling HJURP using ab175577 at a 1/200 dilution (green), followed by washing, antifading and DAPI staining (blue).
This product has been referenced in:
- Li L et al. Increased Expression of Holliday Junction-Recognizing Protein (HJURP) as an Independent Prognostic Biomarker in Advanced-Stage Serous Ovarian Carcinoma. Med Sci Monit 24:3050-3055 (2018). Read more (PubMed: 29743473) »