The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Predicted molecular weight: 84 kDa.
Use at an assay dependent concentration.
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
Nucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.
Immunohistochemical analysis of frozen cytospin preparations of HEK293T cells expressing HJURP (left and right panel) or A-MYB (central panel) as negative control. The left and central panels were incubated with ab175577, labeling HJURP. The right panel was incubated with an antibody against the expression vector itself (positive control).
Western blot - Anti-HJURP antibody [HOPE169E] (ab175577)
All lanes : Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470) at 1/100 dilution
Lane 1 : Human kidney lysate Lane 2 : Human colon lysate Lane 3 : K562 cell lysate Lane 4 : Daudi cell lysate Lane 5 : Raji cell lysate
Li L et al. Increased Expression of Holliday Junction-Recognizing Protein (HJURP) as an Independent Prognostic Biomarker in Advanced-Stage Serous Ovarian Carcinoma. Med Sci Monit24:3050-3055 (2018).
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