Overview

  • Product name
    Anti-HJURP antibody [HOPE169E]
    See all HJURP primary antibodies
  • Description
    Mouse monoclonal [HOPE169E] to HJURP
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (proprietary-tag) corresponding to Human HJURP aa 1-748.
    Database link: Q8NCD3

  • Positive control
    • K562, Daudi and Raji cell lysates; Human kidney lysate; HEK293T cells transfected with HJURP; Human tonsil, tonsil epithelium, thymus and testicle tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    HOPE169E
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Predicted molecular weight: 84 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/200.

Target

  • Relevance
    Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
  • Cellular localization
    Nucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.
  • Database links
  • Alternative names
    • 14-3-3-associated AKT substrate antibody
    • FAKTS antibody
    • fetal liver expressing gene 1 antibody
    • Fetal liver-expressing gene 1 protein antibody
    • FLEG1 antibody
    • hFLEG1 antibody
    • HJURP antibody
    • Holliday junction recognition protein antibody
    • Up-regulated in lung cancer 9 antibody
    • URLC9 antibody
    see all

Images

  • Immunohistochemical analysis of frozen cytospin preparations of HEK293T cells expressing HJURP (left and right panel) or A-MYB (central panel) as negative control. The left and central panels were incubated with ab175577, labeling HJURP. The right panel was incubated with an antibody against the expression vector itself (positive control).

  • All lanes : Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470) at 1/100 dilution

    Lane 1 : Human kidney lysate
    Lane 2 : Human colon lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Daudi cell lysate
    Lane 5 : Raji cell lysate

    Lysates/proteins at 100 µg per lane.

    Predicted band size: 84 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil, tonsil epithelium, thymus and testicle tissues, labeling HJURP using ab175577 at a 1/400 dilution.

  • Immunofluorescence analysis of Human tonsil tissue, labeling HJURP using ab175577 at a 1/200 dilution (green), followed by washing, antifading and DAPI staining (blue).

References

This product has been referenced in:
  • Li L  et al. Increased Expression of Holliday Junction-Recognizing Protein (HJURP) as an Independent Prognostic Biomarker in Advanced-Stage Serous Ovarian Carcinoma. Med Sci Monit 24:3050-3055 (2018). Read more (PubMed: 29743473) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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