Key features and details
- Rabbit polyclonal to HKDC1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-HKDC1 antibody
See all HKDC1 primary antibodies
DescriptionRabbit polyclonal to HKDC1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant fragment within Human HKDC1 (internal sequence). The exact sequence is proprietary.
Database link: Q2TB90
- WB: HEK-293T whole cell extract. IHC-P: Mouse duodenum tissue; Rat brain tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 103 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
PathwayCarbohydrate metabolism; hexose metabolism.
Sequence similaritiesBelongs to the hexokinase family.
- Information by UniProt
- BC016235 antibody
- Hexokinase domain containing 1 antibody
- Hexokinase domain-containing protein 1 antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HKDC1 (green) using ab228729 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
Anti-HKDC1 antibody (ab228729) at 1/2000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Predicted band size: 103 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded mouse duodenum tissue stained for HKDC1 using ab228729 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat brain tissue stained for HKDC1 using ab228729 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228729 has been referenced in 1 publication.
- Chen X et al. PGC1ß Regulates Breast Tumor Growth and Metastasis by SREBP1-Mediated HKDC1 Expression. Front Oncol 9:290 (2019). PubMed: 31058090