Overview

  • Product name
  • Description
    Rabbit polyclonal to HKDC1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human HKDC1 (internal sequence). The exact sequence is proprietary.
    Database link: Q2TB90

  • Positive control
    • WB: HEK-293T whole cell extract. IHC-P: Mouse duodenum tissue; Rat brain tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 103 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HKDC1 (green) using ab228729 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Anti-HKDC1 antibody (ab228729) at 1/2000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

    Predicted band size: 103 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse duodenum tissue stained for HKDC1 using ab228729 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat brain tissue stained for HKDC1 using ab228729 at 1/500 dilution in immunohistochemical analysis.

References

This product has been referenced in:
See 1 Publication for this product

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