Recombinant
RabMAb

Recombinant Anti-HKDC1 antibody [EPR19835] - BSA and Azide free (ab228817)

Overview

  • Product name
    Anti-HKDC1 antibody [EPR19835] - BSA and Azide free
    See all HKDC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR19835] to HKDC1 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human HKDC1 aa 1-500. The exact sequence is proprietary.
    Database link: Q2TB90

  • Positive control
    • WB: HeLa whole cell lysate.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab228817 is a PBS-only buffer format of ab209846. Please refer to ab209846 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
IP Use at an assay dependent concentration.

Target

Images

  • HKDC1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab209846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209846 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg (Input).

    Lane 2: ab209846 IP in HeLa whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209846 in HeLa whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209846).

  • All lanes : Anti-HKDC1 antibody [EPR19835] (ab209846) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HKDC1-knockdown HeLa cell lysate
    Lane 3 : Hexokinase I-knockdown HeLa cell lysate
    Lane 4 : Hexokinase II-knockdown HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 103 kDa
    Observed band size: 102 kDa
    why is the actual band size different from the predicted?



    Blocking/dilution buffer and concentration: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Hui Jiang, NIBS.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209846).

References

ab228817 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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