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    hl-60-whole-cell-lysate-ab7914.pdf

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HL-60 whole cell lysate (ab7914)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

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Overview

  • Product name

    HL-60 whole cell lysate
  • General notes

    Cell line: HL60 (Human promyelocytic leukemia).
    Growth media: RPMI and 10% FBS (Fetal bovine serum).

     

    HL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: 100% SDS Sample Buffer
  • Concentration information loading...
  • Lysate notes

    HL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Human
    • Other
  • Background

    HL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
HL60 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
Notes
WB
Use at an assay dependent concentration.

HL60 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab7914? Please let us know so that we can cite the reference in this datasheet.

ab7914 has been referenced in 1 publication.

  • Manzel LJ  et al. Regulation of Bacteria-Induced Intercellular Adhesion Molecule-1 by CCAAT/Enhancer Binding Proteins. Am J Respir Cell Mol Biol : (2008). PubMed: 18703796

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Our customer would like to purchase positive control for ab102932.

So I searched ab7914. Is it suitable for her purpose?

Check this please.


Best regards

Read More

Abcam community

Verified customer

Asked on Mar 08 2013

Answer

Thank you for contacting us.

The product ab7914 can be used with ab102932.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 08 2013

Question

この製品の細胞はどのぐらい培養したものでしょうか。

Read More

Abcam community

Verified customer

Asked on Jan 13 2009

Answer

いつもお世話になっております。

先日お電話いただきましてありがとうございます。

早速ですが、製造元より回答が届きましたので、お伝えいたします。

HL60細胞を2-4週間(ログフェーズ成長)で培養してから、ライセートの製品化にしております。

上記の情報がお客様に役立つことを願っております。

また他にご質問等がございましたら、お気軽にご連絡ください。

Read More

Abcam Scientific Support

Answered on Jan 13 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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