Overview

  • Product name
    Anti-HLA A2 antibody [BB7.2] (FITC)
    See all HLA A2 primary antibodies
  • Description
    Mouse monoclonal [BB7.2] to HLA A2 (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Ab27728 recognises the human HLA A2 histocompatability antigen.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Papain solubilised HLA A2 (Human).

  • Epitope
    The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy terminus of the alpha 2 helix and a turn on one of the underlying beta strands.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Ab27728 was purified from tissue culture supernatant, and prepared by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    BB7.2
  • Myeloma
    NS1
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

Use 10 µl of the suggested working dilution to label 106 cells or 100µl whole blood.

 

 

 

ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.
  • Cellular localization
    Membrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • Antigen presenting molecule antibody
    • HLA A antibody
    • HLA A histocompatibility type antibody
    • HLA antibody
    • HLA class I antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A2 alpha chain antibody
    • HLA-A antibody
    • HLAA antibody
    • Human leukocyte antigen antibody
    • Leucocyte antigen class I antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • Major histocompatibility complex class I antibody
    • MHC class I antigen [precursor] antibody
    • MHC class I antigen A antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A2 antibody
    • MHC class I antigen HLA A antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC leukocyte antigen antibody
    see all

Images

  • Ab27728, undiluted or at a dilution of 1/10, staining HLA A2 in human peripheral blood monocytes by FACS.

References

This product has been referenced in:
  • Li D  et al. Engineering chimeric human and mouse major histocompatibility complex (MHC) class I tetramers for the production of T-cell receptor (TCR) mimic antibodies. PLoS One 12:e0176642 (2017). Flow Cyt . Read more (PubMed: 28448627) »
  • Straetemans T  et al. Recurrence of melanoma following T cell treatment: continued antigen expression in a tumor that evades T cell recruitment. Mol Ther 23:396-406 (2015). Read more (PubMed: 25363716) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have any empirical data to indicate if this clone is specific just to the HLA-A201 allele or if detects other alleles.

http://www.ncbi.nlm.nih.gov/pubmed/19265143

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for filling out the questionnaire. Your protocol looks good. Do you incubate the cells with the antibody in the dark? FITC is sensitive to photo-bleaching. The only other recommendation I can make would be to try BSA instead of FBS for blocking, in case there are interactions with fetal serum proteins other than BSA that are blocking binding of the antibody to the HLA A2. The PBS-only control is a good idea but you may be losing the antibody to hydrophobic interactions with the tube you incubate in when you leave out blocking protein. The BSA is admittedly a long-shot solution. If you feel photo-bleaching is not a problem, then I can send you a free-of-charge replacement vial, or a refund or credit. Let me know what you would like.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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