Anti-HLA-A+HLA-B+HLA-C antibody (ab200740)

Overview

  • Product name

    Anti-HLA-A+HLA-B+HLA-C antibody
  • Description

    Rabbit polyclonal to HLA-A+HLA-B+HLA-C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human HLA-A+HLA-B+HLA-C aa 243-288.

  • Positive control

    • A549, sp2/0 & H9C2 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.0975% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purity is > 95% (by SDS-PAGE).
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab200740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.

Target

Images

  • All lanes : Anti-HLA-A+HLA-B+HLA-C antibody (ab200740)

    Lane 1 : A549 whole cell lysate
    Lane 2 : sp2/0 whole cell lysate
    Lane 3 : H9C2 whole cell lysate

    Predicted band size: 41 kDa

References

ab200740 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (tumor)
Gel Running Conditions
Reduced Denaturing (4-15 %)
Loading amount
50 µg
Specification
tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Miss. Sylvie Colin

Verified customer

Submitted Oct 20 2017

Question
Answer

The recommended dilution range for ab200740 in western blot application is 1:500-1:1000. The antibody dilution used in the blot image on the website was 1:500.

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