Key features and details
- Rabbit polyclonal to HLA B
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HLA B antibody
See all HLA B primary antibodies
DescriptionRabbit polyclonal to HLA B
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment (His-tag) corresponding to Human HLA B aa 26-309. (Expressed in E.coli)
SHSMRYFHTSVSRPGRGEPRFITVGYVDDTLFVRFDSDAASPREEPRAPW IEQEGPEYWDRETQICKAKAQTDREDLRTLLRYYNQSEAGSHTLQNMYGC DVGPDGRLLRGYHQDAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAAR VAEQLRAYLEGECVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATL RCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPS GEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPIV
Database link: P03989
- IHC-P: Human liver cancer, prostate, kidney, stomach, cerebrum and liver tissue. WB: Recombinant human HLA B protein; HeLa cell lysate; Pig spleen and lymph node tissue lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.|
FunctionInvolved in the presentation of foreign antigens to the immune system.
Sequence similaritiesBelongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
modificationsPolyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system.
- Information by UniProt
- 1B07_HUMAN antibody
- AS antibody
- B-4901 antibody
Anti-HLA B antibody (ab232741) at 5 µg/ml + Recombinant human HLA B protein
HRP-Linked Guinea pig anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 40 kDa
All lanes : Anti-HLA B antibody (ab232741) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Pig spleen tissue lysate
Lane 3 : Pig lymph node tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 40 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for HLA B using ab232741 at 20 ug/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for HLA B using ab232741 at 20 ug/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach tissue stained for HLA B using ab232741 at 20 ug/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human kidney tissue stained for HLA B using ab232741 at 20 ug/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate tissue stained for HLA B using ab232741 at 20 ug/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for HLA B using ab232741 at 20 ug/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232741 has not yet been referenced specifically in any publications.