Key features and details
- Mouse monoclonal [HLA-ABC-m3] to HLA B27 (FITC)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Product nameAnti-HLA B27 antibody [HLA-ABC-m3] (FITC)
See all HLA B27 primary antibodies
DescriptionMouse monoclonal [HLA-ABC-m3] to HLA B27 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Immune complex precipitated from HLA B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
Purification notesab23840 is a purified IgG prepared by ion exchange chromatography.
Primary antibody notesab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
Our Abpromise guarantee covers the use of ab23840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceInvolved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
Cellular localizationSingle-pass type 1 membrane protein
- HLAB antibody
- Leukocyte antigen class I B antibody
- Lymphocyte antigen antibody
ab23840 has been referenced in 4 publications.
- Chen LG et al. Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways. Oncol Lett 12:3344-3350 (2016). Flow Cyt . PubMed: 27900002
- de Jong MA et al. TNF-alpha and TLR agonists increase susceptibility to HIV-1 transmission by human Langerhans cells ex vivo. J Clin Invest 118:3440-3452 (2008). ICC/IF ; Human . PubMed: 18776939
- Orr K et al. Utilization of commercial antisera and flow cytometry in HLA-B27 typing. Cytometry 18:17-20 (1994). PubMed: 8082482
- Trapani JA et al. Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3. Hum Immunol 7:205-16 (1983). PubMed: 6604046