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Dear Technical Team,
Would you please let me know urgently what is the test #s canAnti-HLA B27 antibody [HLA-ABC-m3] (FITC) (ab23840) do? Urgent please.
Asked on Apr 23 2012
Thank you for your enquiry.
The number of smaples can we worked out from the information on the datasheet, 250ul of antibody is provided. The amountof tess in can do will very much depend on the dilution of antibody that you use, which may require some optimization.
The datasheet recommends to use 10µl for 106 cells
So if each sample contains 10e6 cells, you will use 10 ul for each sample.
250 ul/ 10 µl = 25 samples
However, please note the amount per sample is a recommendation only, and may require some optimization.
The datasheet recommend to 'Use at an assay dependent dilution (PMID 18776939).'
You may need to optimize the dilution used. I can suggest to consider a titration experiment using the antibody at 1:20, 1:100, 1:250 and 1:500 to see which provides the optimal results. The dilution used will affect the number of samples you can use. As an example:
Use the antibody at 1:250
100 ul per slide
250 ul x 1:100 = 25000 ul = 25 mls
25 mls / 100 ul per slide = 250 samples
I hope this will be helpful to you. If you have any further questions, please do not hesitate to contactme.
Answered on Apr 23 2012