Overview

  • Product name

    Anti-HLA B7 antibody [BB7.1]
    See all HLA B7 primary antibodies
  • Description

    Mouse monoclonal [BB7.1] to HLA B7
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Monkey
  • Immunogen

    Full length protein (Human). Papain solubilised HLA-A2, B7

  • General notes


    Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing.
  • Clonality

    Monoclonal
  • Clone number

    BB7.1
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

Neat - 1/10.

 

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa. Hundreds of HLA B alleles have been described.
  • Cellular localization

    Cell Membrane, single pass type I membrane protein.
  • Database links

  • Alternative names

    • Abacavir hypersensitivity, susceptibility to antibody
    • Drug-induced liver injury due to flucloxacillin antibody
    • HLA B antibody
    • HLA class I histocompatibility antigen B 7 alpha chain antibody
    • HLA class I histocompatibility antigen, B-13 alpha chain antibody
    • HLA class I histocompatibility antigen, B-14 alpha chain antibody
    • HLA class I histocompatibility antigen, B-15 alpha chain antibody
    • HLA class I histocompatibility antigen, B-18 alpha chain antibody
    • HLA class I histocompatibility antigen, B-27 alpha chain antibody
    • HLA class I histocompatibility antigen, B-35 alpha chain antibody
    • HLA class I histocompatibility antigen, B-37 alpha chain antibody
    • HLA class I histocompatibility antigen, B-38 alpha chain antibody
    • HLA class I histocompatibility antigen, B-40 alpha chain antibody
    • HLA class I histocompatibility antigen, B-41 alpha chain antibody
    • HLA class I histocompatibility antigen, B-42 alpha chain antibody
    • HLA class I histocompatibility antigen, B-44 alpha chain antibody
    • HLA class I histocompatibility antigen, B-45 alpha chain antibody
    • HLA class I histocompatibility antigen, B-46 alpha chain antibody
    • HLA class I histocompatibility antigen, B-47 alpha chain antibody
    • HLA class I histocompatibility antigen, B-48 alpha chain antibody
    • HLA class I histocompatibility antigen, B-49 alpha chain antibody
    • HLA class I histocompatibility antigen, B-50 alpha chain antibody
    • HLA class I histocompatibility antigen, B-51 alpha chain antibody
    • HLA class I histocompatibility antigen, B-52 alpha chain antibody
    • HLA class I histocompatibility antigen, B-53 alpha chain antibody
    • HLA class I histocompatibility antigen, B-54 alpha chain antibody
    • HLA class I histocompatibility antigen, B-55 alpha chain antibody
    • HLA class I histocompatibility antigen, B-56 alpha chain antibody
    • HLA class I histocompatibility antigen, B-57 alpha chain antibody
    • HLA class I histocompatibility antigen, B-58 alpha chain antibody
    • HLA class I histocompatibility antigen, B-59 alpha chain antibody
    • HLA class I histocompatibility antigen, B-67 alpha chain antibody
    • HLA class I histocompatibility antigen, B-7 alpha chain antibody
    • HLA class I histocompatibility antigen, B-73 alpha chain antibody
    • HLA class I histocompatibility antigen, B-78 alpha chain antibody
    • HLA class I histocompatibility antigen, B-8 alpha chain antibody
    • HLA class I histocompatibility antigen, B-81 alpha chain antibody
    • HLA class I histocompatibility antigen, B-82 alpha chain antibody
    • HLAB antibody
    • Leukocyte antigen class I B antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I B antibody
    • MHC class I antigen B 7 antibody
    • Synovitis, chronic, susceptibility to antibody
    see all

Images

  • Overlay histogram showing Jurkat cells stained with ab20169 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (a20169, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

The exact molecular weight of these antibodies is not known, however, most mammalian antibodies have a total molecular weight of approximately 150kDa. This is broken down into two heavy chains of approximately 50kDa each and to light chains of approximately 25kDa each.

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Answer

Thank you for contacting Abcam. According to the information on the website, both ab20169 and ab7855 should recognize a protein that has a molecular weight of approximately 45kDa. If there is anything else I can help you with, please le me know.

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