Key features and details
- Mouse monoclonal [CATA-1] to HLA B7
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-HLA B7 antibody [CATA-1]
See all HLA B7 primary antibodies
DescriptionMouse monoclonal [CATA-1] to HLA B7
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human HLA B7. Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2 and DRw10.
Database link: P01889
- IHC-P: Human tonsil tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from bioreactor concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab236491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Incubate with primary antibody for 30 minutes at RT.
RelevanceHLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa. Hundreds of HLA B alleles have been described.
Cellular localizationCell Membrane, single pass type I membrane protein.
- Abacavir hypersensitivity, susceptibility to antibody
- Drug-induced liver injury due to flucloxacillin antibody
- HLA B antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236491 has not yet been referenced specifically in any publications.