Recombinant Anti-HLA B7 antibody [EPR2623(2)] - BSA and Azide free (ab247757)
![Western blot - Anti-HLA B7 antibody [EPR2623(2)] - BSA and Azide free (ab247757)](https://www.abcam.com/ps/products/247/ab247757/Images/ab247757-402706-anti-hla-b7-antibody-epr2623-2-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-HLA B7 antibody [EPR2623(2)] - BSA and Azide free (ab247757)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2623(2)] to HLA B7 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-HLA B7 antibody [EPR2623(2)] - BSA and Azide free
See all HLA B7 primary antibodies -
Description
Rabbit monoclonal [EPR2623(2)] to HLA B7 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HLA B7 aa 1-100 (extracellular). The exact sequence is proprietary.
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General notes
ab247757 is the carrier-free version of ab109089 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247757 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2623(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab247757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.
|
Target
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Relevance
HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa. Hundreds of HLA B alleles have been described. -
Cellular localization
Cell Membrane, single pass type I membrane protein. -
Database links
- Entrez Gene: 3106 Human
- Omim: 142830 Human
- SwissProt: P01889 Human
- Unigene: 77961 Human
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Alternative names
- Abacavir hypersensitivity, susceptibility to antibody
- Drug-induced liver injury due to flucloxacillin antibody
- HLA B antibody
see all
Images
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Western blot - Anti-HLA B7 antibody [EPR2623(2)] - BSA and Azide free (ab247757)All lanes : Anti-HLA B7 antibody [EPR2623(2)] (ab109089) at 1/1000 dilution
Lane 1 : Jurkat Ramos cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : TF1 cell lysate
Lane 4 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDaThis data was developed using ab109089, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA B7 antibody [EPR2623(2)] - BSA and Azide free (ab247757)](https://www.abcam.com/ps/products/247/ab247757/Images/ab247757-1-anti-hla-b7-antibody-epr2623-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA B7 antibody [EPR2623(2)] - BSA and Azide free (ab247757)This data was developed using ab109089, the same antibody clone in a different buffer formulation.ab109089 at 1/250 dilution staining HLA B7 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-HLA B7 antibody [EPR2623(2)] - BSA and Azide free (ab247757)](https://www.abcam.com/ps/products/247/ab247757/Images/ab247757-3-benefits-of-recombinant-antibodies.png)
Anti-HLA B7 antibody [EPR2623(2)] - BSA and Azide free (ab247757)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247757 has not yet been referenced specifically in any publications.
