Anti-HLA-C antibody (ab232903)
Key features and details
- Rabbit polyclonal to HLA-C
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-HLA-C antibody
See all HLA-C primary antibodies -
Description
Rabbit polyclonal to HLA-C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human HLA-C aa 1-350. (Expressed in E.coli).
Database link: P10321 -
Positive control
- WB: Recombinant human HLA-C protein; Human lymphocyte lysate; A431 cell lysate; Pig liver lysate. IHC-P: Human lung cancer, liver, kidney, prostate, skin cancer and stomach tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232903 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 3107 Human
- Omim: 142840 Human
- SwissProt: P04222 Human
- SwissProt: P10321 Human
- SwissProt: Q07000 Human
- Unigene: 656020 Human
- Unigene: 743218 Human
- Unigene: 77961 Human
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Alternative names
- 1C07_HUMAN antibody
- Cw-7 alpha chain antibody
- D6S204 antibody
see all
Images
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Anti-HLA-C antibody (ab232903) at 2 µg/ml + Recombinant human HLA-C protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 41 kDa -
All lanes : Anti-HLA-C antibody (ab232903) at 2 µg/ml
Lane 1 : Human lymphocyte lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : Pig liver lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 41 kDa -
Paraffin-embedded human lung cancer tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human liver tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human kidney tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human prostate tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human skin cancer tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human stomach tissue stained for HLA-C using ab232903 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232903 has not yet been referenced specifically in any publications.