For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    hla-class-1-abc-antibody-epr22172-ab225636.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity MHC Class I
Share by email
RecombinantRabMAb

Recombinant Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Western blot - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunoprecipitation - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
  • Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22172] to HLA Class 1 ABC
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Conjugation
Product image
Alkaline phosphatase Conjugation Kit - Lightning-Link® (ab102850)
Primary
Product image
Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-HLA Class 1 ABC antibody [EPR22172]
    See all HLA Class 1 ABC primary antibodies
  • Description

    Rabbit monoclonal [EPR22172] to HLA Class 1 ABC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: THP-1 and Raji cells. Flow Cyt: Raji and THP-1 cells. IHC-P: Human colonic carcinoma, stomach and liver tissue. WB: Raji, THP-1, HL-60, Jurkat, HepG2, TF-1 and Ramos whole cell lysate. Human tonsil. Recombinant human HLA-A protein (aa24-aa365). Recombinant human HLA-C protein (aa25-aa308). IP: HLAB in THP-1 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22172
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I

Associated products

  • Alternative Versions

    • Anti-HLA Class 1 ABC antibody [EPR22172] - BSA and Azide free (ab239788)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunohistochemistry reagents

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
Flow Cyt
1/600.
IP
1/30.
Notes
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
Flow Cyt
1/600.
IP
1/30.

Target

  • Relevance

    HLA-ABC antigen is present on most nucleated cells of humans and Old World monkeys. HLA CLass I is involved in the presentation of foreign antigens to the immune system.
  • Cellular localization

    Cell Membrane; Single-pass type I membrane protein
  • Database links

    • Entrez Gene: 3105 Human
    • Entrez Gene: 3106 Human
    • Entrez Gene: 3107 Human
    • SwissProt: P01889 Human
    • SwissProt: P30443 Human
    • SwissProt: P30499 Human
    • Alternative names

      • Antigen Presenting Molecule antibody
      • Cw*1701 antibody
      • D6S204 antibody
      • FLJ27082 antibody
      • HLA A antibody
      • HLA B antibody
      • HLA C antibody
      • HLA class 1 A antibody
      • HLA class 1 B antibody
      • HLA class 1 C antibody
      • HLA JY3 antibody
      • HLAA antibody
      • HLAB antibody
      • HLAC antibody
      • HLC C antibody
      • Human Leucocyte Antigen C antibody
      • Major Histocompatibility Complex Class I A antibody
      • Major Histocompatibility Complex Class I B antibody
      • Major Histocompatibility Complex Class I C antibody
      • Major histocompatibility complex, class I, A + B + C antibody
      • MHC class I antigen A*1 antibody
      • MHC Class I Antigen antibody
      • MHC class I antigen B*7 antibody
      • MHC class I antigen Cw*1 antibody
      • MHC class I HLA A antibody
      • MHC class I HLA B antibody
      • MHC class I HLA C antibody
      • MHC HLA ABC antibody
      • PSORS1 antibody
      see all

    Images

    • Western blot - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Western blot - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      All lanes : Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636) at 1/1000 dilution

      Lane 1 : Recombinant human HLA-A protein (aa24-aa365) 10ng
      Lane 3 : Recombinant human HLA-C protein (aa25-aa308) 10ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 40 kDa



      This antibody has cross reaction with human HLA-A and HLA-C.# Recombinant human HLA-A protein was purchased from abnova company (H00003105-P01).

      Recombinant human HLA-C protein was purchased from novusbio company (NBP2-23210).

    • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunofluorescent analysis of 100% methanol-fixed THP-1 (human monocytic leukemia cell line) cells labeling HLAB with ab225636 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in THP-1 cells. The nuclear counterstain is DAPI (blue).
      Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
      The negative control is the secondary antibody only.

      Methanol is recommended for fixation as weak signal was observed using 4% PFA.

    • Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cells labeling HLAB with ab225636 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling HLAB with ab225636 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining on lymph nodule of human stomach (PMID:25294906) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Western blot - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Western blot - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      All lanes : Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636) at 1/1000 dilution

      Lane 1 : Raji (human Burkitt's lymphoma cell line), whole cell lysate
      Lane 2 : THP-1 (human monocytic leukemia cell line), whole cell lysate
      Lane 3 : HL-60 (human promyelocytic leukemia cell line), whole cell lysate
      Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood), whole cell lysate
      Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line), whole cell lysate
      Lane 6 : TF-1 (human bone marrow erythroleukemia cell line), whole cell lysate
      Lane 7 : Ramos (human Burkitt's lymphoma cell line), whole cell lysate
      Lane 8 : Human tonsil

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 40 kDa
      Observed band size: 40 kDa


      Exposure time: 26 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Weak reactive bands of undetermined proteins are observed in addition to the target band.

       

    • Immunoprecipitation - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunoprecipitation - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      HLAB was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia cell line) whole cell lysate with ab225636 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225636 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
      Lane 1: THP-1 whole cell lysate 10 µg (Input).
      Lane 2: ab225636 IP in THP-1 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225636 in THP-1 whole cell lysate.
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 3 seconds.

    • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunocytochemistry/ Immunofluorescence - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) cells labeling HLAB with ab225636 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Raji cells. The nuclear counterstain is DAPI (blue).
      Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
      The negative control is the secondary antibody only.

      Methanol is recommended for fixation as weak signal was observed using 4% PFA.

    • Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling HLAB with ab225636 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HLAB with ab225636 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on hepatic sinus endothelium of human liver (PMID: 26963506) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

      Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue labeling HLAB with ab225636 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining on tumor cells of human colonic carcinoma (PMID: 26310568, PMID: 17014712) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)
      Anti-HLA Class 1 ABC antibody [EPR22172] (ab225636)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab225636? Please let us know so that we can cite the reference in this datasheet.

    ab225636 has been referenced in 1 publication.

    • Shen R  et al. Influence of oncogenic mutations and tumor microenvironment alterations on extranodal invasion in diffuse large B-cell lymphoma. Clin Transl Med 10:e221 (2020). PubMed: 33252851

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab225636.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.