Key features and details
- Rabbit polyclonal to HLA Class I A1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-HLA Class I A1 antibody
DescriptionRabbit polyclonal to HLA Class I A1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human HLA Class I A1 aa 35-285.
SVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYW DQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFL RGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYL EGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYP AEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCH V
Database link: P30443
- THP1, HeLa, DU145, SW480 and HepG2 cell extracts; Mouse liver extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 41 kDa.|
|IHC-P||1/50 - 1/200.|
|ICC/IF||Use at an assay dependent concentration.|
Cellular localizationMembrane; Single-pass type I membrane protein
- antigen presenting molecule antibody
- FLJ26655 antibody
- HLA class I histocompatibility antigen, A-1 alpha chain antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling HLA Class I A1 with ab185706 at 1/500. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling HLA Class I A1 with ab185706 at 1/500. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human neurilemmoma tissue labelling HLA Class I A1 with ab185706 at 1/500. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling HLA Class I A1 with ab185706 at 1/500. Magnification: 200x.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185706. Blue DAPI for nuclear staining.
All lanes : Anti-HLA Class I A1 antibody (ab185706) at 1/500 dilution
Lane 1 : THP1 cell extract
Lane 2 : HeLa cell extract
Lane 3 : DU145 cell extract
Lane 4 : SW480 cell extract
Lane 5 : HepG2 cell extract
Lane 6 : Mouse liver extract
Predicted band size: 41 kDa
ab185706 has been referenced in 1 publication.
- Chen X et al. Decoy receptor-3 regulates inflammation and apoptosis via PI3K/AKT signaling pathway in coronary heart disease. Exp Ther Med 17:2614-2622 (2019). PubMed: 30906453