Anti-HLA Class I antibody [MEM-123] (ab2217)
Key features and details
- Mouse monoclonal [MEM-123] to HLA Class I
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG3
Related conjugates and formulations
Overview
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Product name
Anti-HLA Class I antibody [MEM-123]
See all HLA Class I primary antibodies -
Description
Mouse monoclonal [MEM-123] to HLA Class I -
Host species
Mouse -
Specificity
This antibody reacts with all human classical MHC Class I molecules in native cell-surface forms as well as with human HLA-G cDNA transfected cells. This antibody completely blocks the binding of pan MHC antibody (ab7855) but does not block binding of anti-HLA-G(ab7758/7904) to surface-expressed HLA-G. -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to HLA Class I. COS-7 cells transfected with human CD48
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-123 -
Myeloma
unknown -
Isotype
IgG3 -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use a concentration of 4 µg/ml.
ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use a concentration of 4 µg/ml. ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P04439 Human
- SwissProt: P13747 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Images
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Flow Cytometry analysis of human peripheral blood cells labeling HLA Class I with Anti-HLA Class I antibody [MEM-123] (ab2217).
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ICC/IF image of ab2217 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2217, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing Jurkat cells stained with ab2217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2217, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab2217 has been referenced in 1 publication.
- Kuželová K et al. NPM1 and DNMT3A mutations are associated with distinct blast immunophenotype in acute myeloid leukemia. Oncoimmunology 11:2073050 (2022). PubMed: 35558161