Anti-HLA Class I antibody [MEM-123] (ab2217)
![Flow Cytometry - Anti-HLA Class I antibody [MEM-123] (ab2217)](https://www.abcam.com/ps/products/2/ab2217/Images/ab2217-3-ab2217FC.jpg "Flow Cytometry - Anti-HLA Class I antibody [MEM-123] (ab2217)")
Key features and details
- Mouse monoclonal [MEM-123] to HLA Class I
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human, Monkey
- Isotype: IgG3
Overview
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Product name
Anti-HLA Class I antibody [MEM-123]
See all HLA Class I primary antibodies -
Description
Mouse monoclonal [MEM-123] to HLA Class I -
Host species
Mouse -
Specificity
This antibody reacts with all human classical MHC Class I molecules in native cell-surface forms as well as with human HLA-G cDNA transfected cells. This antibody completely blocks the binding of pan MHC antibody (ab7855) but does not block binding of anti-HLA-G(ab7758/7904) to surface-expressed HLA-G. -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human, Monkey -
Immunogen
Tissue/ cell preparation. COS-7 cells transfected with human CD48.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-123 -
Myeloma
unknown -
Isotype
IgG3 -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use a concentration of 0.5 - 4 µg/ml. ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P01891 Human
- SwissProt: P01892 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Images
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Flow Cytometry - Anti-HLA Class I antibody [MEM-123] (ab2217)Flow Cytometry analysis of human peripheral blood cells labeling HLA Class I with Anti-HLA Class I antibody [MEM-123] (ab2217).
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![Immunocytochemistry/ Immunofluorescence - Anti-HLA Class I antibody [MEM-123] (ab2217)](https://www.abcam.com/ps/products/2/ab2217/Images/HLA-Class-I-Primary-antibodies-ab2217-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HLA Class I antibody [MEM-123] (ab2217)ICC/IF image of ab2217 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2217, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Flow Cytometry - Anti-HLA Class I antibody [MEM-123] (ab2217)](https://www.abcam.com/ps/products/2/ab2217/Images/HLA-Class-I-Primary-antibodies-ab2217-2.jpg)
Flow Cytometry - Anti-HLA Class I antibody [MEM-123] (ab2217)Overlay histogram showing Jurkat cells stained with ab2217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2217, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
References (0)
ab2217 has not yet been referenced specifically in any publications.
