Key features and details
- Mouse monoclonal [MEM-123] to HLA Class I (PE)
- Suitable for: IP, ELISA, Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG3
Product nameAnti-HLA Class I antibody [MEM-123] (PE)
See all HLA Class I primary antibodies
DescriptionMouse monoclonal [MEM-123] to HLA Class I (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Specificityab58998 reacts with all human classical MHC Class I molecules in native cell-surface forms as well as with human HLA G cDNA transfected cells. ab58998 completely blocks binding of classical W6/32 to surface expressed HLA G.
Tested applicationsSuitable for: IP, ELISA, Flow Cytmore details
Species reactivityReacts with: Human
COS7 African green monkey kidney cells
General notesDo not use after expiration date stamped on vial label. Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
The purified antibody was conjugated with R Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
0.2% high grade protease free BSA
Concentration information loading...
Purification notesThe antibody was >95% pure as determined from SDS-PAGE (purified using protein A affinity chromatography prior to conjugation).
Our Abpromise guarantee covers the use of ab58998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution.|
RelevanceHLA CLass I is involved in the presentation of foreign antigens to the immune system.
Cellular localizationPlasma membrane
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
ab58998 has been referenced in 1 publication.
- Toyoshima K et al. Analysis of circulating tumor cells derived from advanced gastric cancer. Int J Cancer 137:991-8 (2015). Flow Cyt ; Human . PubMed: 25622566