Overview

  • Product name

    Anti-HLA Class I antibody [MEM-123] (Phycoerythrin)
    See all HLA Class I primary antibodies
  • Description

    Mouse monoclonal [MEM-123] to HLA Class I (Phycoerythrin)
  • Host species

    Mouse
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity

    ab58998 reacts with all human classical MHC Class I molecules in native cell-surface forms as well as with human HLA G cDNA transfected cells. ab58998 completely blocks binding of classical W6/32 to surface expressed HLA G.
  • Tested applications

    Suitable for: IP, ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    COS7 African green monkey kidney cells

  • General notes

    Do not use after expiration date stamped on vial label. Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

    The purified antibody was conjugated with R Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituent: 0.2% BSA

    0.2% high grade protease free BSA
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    The antibody was >95% pure as determined from SDS-PAGE (purified using protein A affinity chromatography prior to conjugation).
  • Clonality

    Monoclonal
  • Clone number

    MEM-123
  • Isotype

    IgG3
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab58998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

Target

Images

  • Flow Cytometry analysis of K562 cells labeling HLA Class I with Anti-HLA Class I antibody [MEM-123] (Phycoerythrin) (ab58998).

References

This product has been referenced in:

  • Toyoshima K  et al. Analysis of circulating tumor cells derived from advanced gastric cancer. Int J Cancer 137:991-8 (2015). Flow Cyt ; Human . Read more (PubMed: 25622566) »
See 1 Publication for this product

Customer reviews and Q&As

Answer


DISCOUNT CODE: ***
Expiration date: June 22, 2012

This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for ab58998 in IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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