Anti-HLA Class I antibody [MEM-147] (ab9090)

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Overview

  • Product name
    Anti-HLA Class I antibody [MEM-147]
    See all HLA Class I primary antibodies
  • Description
    Mouse monoclonal [MEM-147] to HLA Class I
  • Host species
    Mouse
  • Specificity
    Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32 (ab7855).
  • Tested applications
    Suitable for: Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    PHA-activated peripheral blood lymphocytes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Purified from TCS
  • Clonality
    Monoclonal
  • Clone number
    MEM-147
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.
WB 1/100. Use non-reducing conditions.

Target

Images

  • Western blot - Anti-HLA Class I antibody [MEM-147] (ab9090)
    Western blot - Anti-HLA Class I antibody [MEM-147] (ab9090)
    Anti-HLA Class I antibody [MEM-147] (ab9090) at 1/100 dilution + Human Ramos B cell line lysates (1% laurylmaltoside)

    Secondary
    Goat anti-mouse IgG (H+L)-HRP conjugate

    Performed under non-reducing conditions.
  • Flow Cytometry - Anti-HLA Class I antibody [MEM-147] (ab9090)
    Flow Cytometry - Anti-HLA Class I antibody [MEM-147] (ab9090)
    Overlay histogram showing Jurkat cells stained with ab9090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9090, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:
  • Tran TM  et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). Read more (PubMed: 11685454) »
See 1 Publication for this product

Publishing research using ab9090? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

I am looking for an antibody for FACS analysis that recognizes HLA-A, B or C but not the B2 microglobulin portion of the HLA Class I antigen. I need to know if the actual HLA-A, B or C molecule is expressed on the outside of the cell and if the anitbody recognizes the B2 microglobulin part of the cell, I can't tell if just the B2 microglobulin is expressed without the HLA-A, B or C molecule or not. I am working with stem cells and the current FACS antibody I'm using recognizes the B2 microglobulin portion as well as A, B and C. So even if the cell is only expressing the B2 microglobulin, there would be a very strong postive result, but the cell does not actually express functional HLA-A, B or C. Thank you for your assistance in this matter.

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Answer

Thank you for your enquiry. For this particular antibody, we unfortunately do not have this information. Our source for ab9090 has also said that they think that a pan MHC antibody that recognizes only the free heavy chains on the surface (where it is often complexed with b2m) is not commercially available anywhere. If you have any additional questions, please contact us again.

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Question

I am looking for an anti HLA classI monoclonal antibody of the IgM isotype. Please, could you let me know if there is one IgM isotype among yours ab 2217 (MEM 123), ab 9090 (MEM 147) or ab 3975 (MEM 81).

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Answer

Thank you for your email. Ab2217 is isotype IgG3; ab9090 is isotype IgG1; and ab3975 is isotype IgG1. You may also find the isotype information on our online datasheets. If you have any additional questions, please contact us again.

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Question

Customer is interested in using this antibody (ab9090) in IP and WB, and would like to know if it cross-reacts with HLA-G or whether it blocks any other HLA G antibodies. Any information would be greatly appreciated. Thanks!

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Answer

The supplier gave me the following information regarding your question. If you have any additional questions, please contact us again. The antibody MEM-147 (ab9090) does not react with HLA-G1 overexpressed in transfectants. It can be used for IP experiments-it recognizes most HLA-A,B,C in native conformation. In western blots-NON REDUCED NON BOILED samples only, but no HLA-A will be detected. For details see: Tran T.M. et al., Immunogenetics 53, 440 (2001) - the comparative study with W6/32 antibody.

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Question

I'm interested in a marker for human cells - identifying cells implanted into rodent brains viewed immunohistochemically. The human cells implanted may take on any phenotype so we are not able to rely simply on something like a human specific anti-nerve specific enolase - we could get human cardiac tissue, connective tissue, blood cells, anything! What is the best choice - or range of choices for anti-human markers in such a situation? Thanks

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Answer

Interesting question, but I'm afraid that we are unable to offer any suggestions. Please let us know if you find a suitable target.

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Question

Hi Abcamteam ! Is this antibody useful for blocking experiments in an MLTC ? Best regards from Mainz Kai Kronfeld

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Answer

As far as we are aware, this antibody has not yet been tested in this application.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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