Overview

  • Product name

    Anti-HLA Class I antibody [MEM-147] (Phycoerythrin)
    See all HLA Class I primary antibodies
  • Description

    Mouse monoclonal [MEM-147] to HLA Class I (Phycoerythrin)
  • Host species

    Mouse
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity

    ab5451 recognises all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. ab52451 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    PHA-activated peripheral blood lymphocytes.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituent: 0.2% BSA

    BSA (high-grade protease free),
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab52451 was purified by Protein G prior to conjugation. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    MEM-147
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 5 µg/ml.

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

References

ab52451 has not yet been referenced specifically in any publications.

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