Anti-HLA Class I antibody [W6/32] (ab22432)

Reviews (1)Q&A (1)References (17)

Overview

  • Product name

    Anti-HLA Class I antibody [W6/32]
    See all HLA Class I primary antibodies

  • Description

    Mouse monoclonal [W6/32] to HLA Class I

  • Host species

    Mouse

  • Specificity

    Recognises an antigenic determinant shared among products of the HLA A, B and C loci. The antibody recognises virtually all nucleated human cells. It recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IP, ELISAmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Cow, Cat, Human

  • Immunogen

    Tissue, cells or virus corresponding to Human HLA Class I. Tissue/ cell preparation of membrane of tonsil cells (Human).

  • Positive control

    • FACS: Human peripheral blood lymphocytes. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    W6/32

  • Myeloma

    NS1/1-Ag4-1

  • Isotype

    IgG2a

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

See Cabrera CM et al reference.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    Images

    • Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)
      Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)

      Flow cytometric analysis of red cell lysed human blood gated on lymphocytes in the presence of Human seroblock using ab22432 at 1/50 dilution (Figure B) compared to anPacific Blue conjugated Mouse IgG2a isotype control (Figure A).

    • Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)
      Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)

      Flow cytometric analysis of red cell lysed human blood gated on lymphocytes using ab22432 at 1/50 dilution (Figure B) compared to an isotype control (Figure A).

    • Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)
      Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)

      Staining of human peripheral blood lymphocytes with ab22432 (red).

    • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class I antibody [W6/32] (ab22432)
      Immunocytochemistry/ Immunofluorescence - Anti-HLA Class I antibody [W6/32] (ab22432)

      ab22432 at a 1/100 dilution staining unpermeabilized HeLa cells (Human epithelial cell line from cervix adenocarcinoma) by Immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 10% serum and incubated with the antibody for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.

      This image is courtesy of an Anonymous Abreview submitted on 12 April 2006.

      See Abreview

    References

    This product has been referenced in:

    • Agak GW  et al. Phenotype and Antimicrobial Activity of Th17 Cells Induced by Propionibacterium acnes Strains Associated with Healthy and Acne Skin. J Invest Dermatol 138:316-324 (2018). Read more (PubMed: 28864077) »
    • Tsuruta M  et al. Bladder cancer-associated cancer-testis antigen-derived long peptides encompassing both CTL and promiscuous HLA class II-restricted Th cell epitopes induced CD4+ T cells expressing converged T-cell receptor genes in vitro. Oncoimmunology 7:e1415687 (2018). Read more (PubMed: 29632734) »
    See all 17 Publications for this product

    Publishing research using ab22432? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I would like to ask if the light chain for the antibody W6/32 is gamma or kappa light chain. Thanks.

    Read More
    Answer

    I'm afraid this has not been tested, so we do not know if it is a gamma or kappa light chain. Sorry I couldn't help you more on this occasion,

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HLA Class I antibody [W6/32]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Cervical carcinoma (HeLa))
    Specification
    Cervical carcinoma (HeLa)
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 12 2006

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