Anti-HLA Class I antibody [W6/32] (ab22432)
![Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)](https://www.abcam.com/ps/products/22/ab22432/Images/ab22432-346693-anti-hla-class-1-w632-antibody-flow-cytometry-human-blood-lymphoctyes.jpg "Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)")
Key features and details
- Mouse monoclonal [W6/32] to HLA Class I
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-HLA Class I antibody [W6/32]
See all HLA Class I primary antibodies -
Description
Mouse monoclonal [W6/32] to HLA Class I -
Host species
Mouse -
Specificity
Recognises an antigenic determinant shared among products of the HLA A, B and C loci. The antibody recognises virtually all nucleated human cells. It recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Chimpanzee, Baboon, Cynomolgus monkey, Macaque monkey, Rhesus monkey, Gorilla
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Immunogen
Tissue, cells or virus corresponding to Human HLA Class I. Purified human tonsil lymphocyte membranes.
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Positive control
- Flow Cyt: Human peripheral blood lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
W6/32 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: P16209 Chimpanzee
- SwissProt: P30375 Gorilla
- SwissProt: O15506 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Images
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Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)
Flow cytometric analysis of red cell lysed human blood gated on lymphocytes in the presence of Human seroblock using ab22432 at 1/50 dilution (Figure B) compared to anPacific Blue conjugated Mouse IgG2a isotype control (Figure A).
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![Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)](https://www.abcam.com/ps/products/22/ab22432/Images/ab22432-346692-anti-hla-class-1-w632-antibody-flow-cytometry-human-blood.jpg)
Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)Flow cytometric analysis of red cell lysed human blood gated on lymphocytes using ab22432 at 1/50 dilution (Figure B) compared to an isotype control (Figure A).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab22432 has been referenced in 28 publications.
- Mahameed M et al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nat Commun 11:1304 (2020). PubMed: 32161259
- Mikkelsen K et al. Carcinoembryonic Antigen (CEA)-Specific 4-1BB-Costimulation Induced by CEA-Targeted 4-1BB-Agonistic Trimerbodies. Front Immunol 10:1791 (2019). PubMed: 31417564
- Grandon B et al. HLA-B27 alters BMP/TGFß signalling in Drosophila, revealing putative pathogenic mechanism for spondyloarthritis. Ann Rheum Dis 78:1653-1662 (2019). PubMed: 31563893
- Agak GW et al. Phenotype and Antimicrobial Activity of Th17 Cells Induced by Propionibacterium acnes Strains Associated with Healthy and Acne Skin. J Invest Dermatol 138:316-324 (2018). PubMed: 28864077
- Tsuruta M et al. Bladder cancer-associated cancer-testis antigen-derived long peptides encompassing both CTL and promiscuous HLA class II-restricted Th cell epitopes induced CD4+ T cells expressing converged T-cell receptor genes in vitro. Oncoimmunology 7:e1415687 (2018). PubMed: 29632734