Overview

  • Product name
    Anti-HLA Class I antibody [W6/32]
    See all HLA Class I primary antibodies
  • Description
    Mouse monoclonal [W6/32] to HLA Class I
  • Host species
    Mouse
  • Specificity
    Recognises an antigenic determinant shared among products of the HLA A, B and C loci. The antibody recognises virtually all nucleated human cells. It recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IP, ELISAmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Cow, Cat, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human HLA Class I. Tissue/ cell preparation of membrane of tonsil cells (Human).

  • Positive control
    • FACS: Human peripheral blood lymphocytes. ICC/IF: HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    W6/32
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

See Cabrera CM et al reference.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    Images

    • Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)
      Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)

      Staining of human peripheral blood lymphocytes with ab22432 (red).

    • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class I antibody [W6/32] (ab22432)
      Immunocytochemistry/ Immunofluorescence - Anti-HLA Class I antibody [W6/32] (ab22432)

      ab22432 at a 1/100 dilution staining unpermeabilized HeLa cells (Human epithelial cell line from cervix adenocarcinoma) by Immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 10% serum and incubated with the antibody for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.

      This image is courtesy of an Anonymous Abreview submitted on 12 April 2006.

      See Abreview

    References

    This product has been referenced in:
    • Agak GW  et al. Phenotype and Antimicrobial Activity of Th17 Cells Induced by Propionibacterium acnes Strains Associated with Healthy and Acne Skin. J Invest Dermatol 138:316-324 (2018). Read more (PubMed: 28864077) »
    • Daniel D  et al. Pathway of apoptosis induced in Jurkat T lymphoblasts by anti-HLA class I antibodies. Hum Immunol 65:189-99 (2004). Read more (PubMed: 15041157) »

    See all 7 Publications for this product

    Publishing research using ab22432? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    2 reviews or Q&A

    10
    Votes: Highest First

    I would like to ask if the light chain for the antibody W6/32 is gamma or kappa light chain. Thanks.

    I'm afraid this has not been tested, so we do not know if it is a gamma or kappa light chain. Sorry I couldn't help you more on this occasion,

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HLA Class I antibody [W6/32]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Cervical carcinoma (HeLa))
    Specification
    Cervical carcinoma (HeLa)
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Apr 12 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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