Anti-HLA Class I antibody [W6/32] (ab22432)
![Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)](https://www.abcam.com/ps/products/22/ab22432/Images/ab22432-346693-anti-hla-class-1-w632-antibody-flow-cytometry-human-blood-lymphoctyes.jpg "Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)")
Key features and details
- Mouse monoclonal [W6/32] to HLA Class I
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-HLA Class I antibody [W6/32]
See all HLA Class I primary antibodies -
Description
Mouse monoclonal [W6/32] to HLA Class I -
Host species
Mouse -
Specificity
Recognises an antigenic determinant shared among products of the HLA A, B and C loci. The antibody recognises virtually all nucleated human cells. It recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Tissue, cells or virus corresponding to Human HLA Class I. Tissue/ cell preparation of membrane of tonsil cells (Human).
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Positive control
- Flow Cyt: Human peripheral blood lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
W6/32 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| All applications |
Cow
Chimpanzee
Baboon
Cynomolgus monkey
Macaque monkey
Rhesus monkey
Gorilla
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: P16209 Chimpanzee
- SwissProt: P30375 Gorilla
- SwissProt: O15506 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Images
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Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)
Flow cytometric analysis of red cell lysed human blood gated on lymphocytes in the presence of Human seroblock using ab22432 at 1/50 dilution (Figure B) compared to anPacific Blue conjugated Mouse IgG2a isotype control (Figure A).
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![Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)](https://www.abcam.com/ps/products/22/ab22432/Images/ab22432-346692-anti-hla-class-1-w632-antibody-flow-cytometry-human-blood.jpg)
Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)Flow cytometric analysis of red cell lysed human blood gated on lymphocytes using ab22432 at 1/50 dilution (Figure B) compared to an isotype control (Figure A).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab22432 has been referenced in 24 publications.
- Agak GW et al. Phenotype and Antimicrobial Activity of Th17 Cells Induced by Propionibacterium acnes Strains Associated with Healthy and Acne Skin. J Invest Dermatol 138:316-324 (2018). PubMed: 28864077
- Tsuruta M et al. Bladder cancer-associated cancer-testis antigen-derived long peptides encompassing both CTL and promiscuous HLA class II-restricted Th cell epitopes induced CD4+ T cells expressing converged T-cell receptor genes in vitro. Oncoimmunology 7:e1415687 (2018). PubMed: 29632734
- Müller M et al. YIP1 family member 4 (YIPF4) is a novel cellular binding partner of the papillomavirus E5 proteins. Sci Rep 5:12523 (2015). PubMed: 26235900
- Turner MA et al. Comparison of the Life Cycles of Genetically Distant Species C and Species D Human Adenoviruses Ad6 and Ad26 in Human Cells. J Virol 89:12401-17 (2015). PubMed: 26423951
- Schmitt J et al. Human Parthenogenetic Embryonic Stem Cell-Derived Neural Stem Cells Express HLA-G and Show Unique Resistance to NK Cell-Mediated Killing. Mol Med 21:185-96 (2015). PubMed: 25811991