Overview

  • Product name
    Anti-HLA Class I antibody [W6/32] (Biotin)
    See all HLA Class I primary antibodies
  • Description
    Mouse monoclonal [W6/32] to HLA Class I (Biotin)
  • Host species
    Mouse
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Cow, Cat, Human, Non human primates
    Does not react with: Rabbit
  • Immunogen

    Tissue, cells or virus corresponding to Human HLA Class I.

  • General notes

    Suitable as a positive control for HLA tissue typing and crossmatching.

    The purified antibody (>95% by SDS-PAGE) is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Size exclusion
  • Primary antibody notes
    Suitable as a positive control for HLA tissue typing and crossmatching.
  • Clonality
    Monoclonal
  • Clone number
    W6/32
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/300.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

References

This product has been referenced in:
  • Tran TM  et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). Read more (PubMed: 11685454) »
  • Kievits F & Ivanyi P Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin. Hum Immunol 20:115-26 (1987). Read more (PubMed: 3316135) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply. To take advantage of the testing discount promotion, you must first purchase the antibody (in this case ab21148) at full price + shipping and handling. You then test the antibody in your sELISA and submit an Abreview on the results that you obtained. These results could be positive or negative, we just want as much information as we can. After you have submitted the Abreview, the testing discount code that I sent will become active and you can then go ahead and use the code to get an antibody free of charge from our catalogue. I hope this helps clarify how the discount program works. You can find all of these details in the link below: https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount Please let me know if there is anything else I can help you with, or if you have any further questions about the testing discount program.

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Answer

Thank you for contacting Abcam. The only monoclonal antibodies we have to HLA Class I antibody [W6/32] (Biotin) are ab21148 and ab110665. As you mentioned neither have been shown to work in ELISA, therefore both are eligible for our testing discount program. Details of this can be found below: https://www.abcam.com/index.htmlpageconfig=resource&rid=11998&viapagetrap=collaborationdiscount If you would like to take advantage of this testing discount program, or if there is anything else I can help you with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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