Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free (ab23755)
![Flow Cytometry - Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free (ab23755)](https://www.abcam.com/ps/products/23/ab23755/Images/ab23755-33810-anti-hla-class-i-antibody-w6-32-flow-cytometry.jpg "Flow Cytometry - Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free (ab23755)")
Key features and details
- Mouse monoclonal [W6/32] to HLA Class I - Low endotoxin, Azide free
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Cow, Cat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free
See all HLA Class I primary antibodies -
Description
Mouse monoclonal [W6/32] to HLA Class I - Low endotoxin, Azide free -
Host species
Mouse -
Specificity
The antibody recognises an antigenic determinant common to HLA A, B and C. In WB, it detects the heavy chain of MHC molecule. We have data to indicate that this antibody may not cross react with Rabbit. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Cow, Cat, Human -
Immunogen
Tissue/ cell preparation - Membrane of human tonsil cells.
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General notes
The antibody recognises virtually all nucleated human cells, it is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
The antibody recognises virtually all nucleated human cells, it is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching. -
Clonality
Monoclonal -
Clone number
W6/32 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab23755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. Paraformaldehyde or methanol fixed cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P01891 Human
- SwissProt: P01892 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Images
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Flow Cytometry - Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free (ab23755)Overlay histogram showing Jurkat cells stained with ab23755 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23755, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Immunocytochemistry/ Immunofluorescence - Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free (ab23755)](https://www.abcam.com/ps/products/23/ab23755/Images/ab23755-33811-anti-hla-class-i-antibody-w6-32-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free (ab23755)ICC/IF image of ab23755 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23755, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (13)
ab23755 has been referenced in 13 publications.
- Okutomi T et al. HIV Reactivation in Latently Infected Cells With Virological Synapse-Like Cell Contact. Viruses 12:N/A (2020). PubMed: 32276457
- Li X et al. Enrichment of CD146+ Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration. Theranostics 9:5105-5121 (2019). PubMed: 31410204
- Wu H et al. IFN-t Mediated Control of Bovine Major Histocompatibility Complex Class I Expression and Functionviathe Regulation of bta-miR-148b/152 in Bovine Endometrial Epithelial Cells. Front Immunol 9:167 (2018). PubMed: 29456541
- Tong M et al. Quantitative Analysis of Cellular Proteome Alterations in CDV-Infected Mink Lung Epithelial Cells. Front Microbiol 8:2564 (2017). PubMed: 29312244
- Zhu Z et al. Interferon-t increases BoLA-I for implantation during early pregnancy in dairy cows. Oncotarget 8:95095-95107 (2017). PubMed: 29221114
