Key features and details
- Rat monoclonal [YTH862.2] to HLA Class I
- Suitable for: Functional Studies, Flow Cyt, IHC-Fr, IP
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-HLA Class I antibody [YTH862.2]
See all HLA Class I primary antibodies
DescriptionRat monoclonal [YTH862.2] to HLA Class I
Specificityab33252 recognises a monomorphic determinant on the human HLA Class I heavy chain. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: Functional Studies, Flow Cyt, IHC-Fr, IPmore details
Species reactivityReacts with: Human
Tissue/ cell preparation: PHA activated human peripheral blood lymphocytes.
Epitopeab33252 reacts with an epitope located in the alpha1 domain of HLA class I molecules.
- Human peripheral blood lymphocytes
Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesab33252 was purified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab33252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.|
|Flow Cyt||1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
RelevanceHLA CLass I is involved in the presentation of foreign antigens to the immune system.
Cellular localizationPlasma membrane
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
ab33252 has not yet been referenced specifically in any publications.