Anti-HLA Class I antibody [YTH862.2] (ab33252)

Reviews (3)Q&A (2)
Flow Cytometry - Anti-HLA Class I antibody [YTH862.2] (ab33252)

    Key features and details

    • Rat monoclonal [YTH862.2] to HLA Class I
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG2b

    Overview

    • Product name

      Anti-HLA Class I antibody [YTH862.2]
      See all HLA Class I primary antibodies

    • Description

      Rat monoclonal [YTH862.2] to HLA Class I

    • Host species

      Rat

    • Specificity

      ab33252 recognises a monomorphic determinant on the human HLA Class I heavy chain. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue/ cell preparation: PHA activated human peripheral blood lymphocytes.

    • Epitope

      ab33252 reacts with an epitope located in the alpha1 domain of HLA class I molecules.

    • Positive control

      • Human peripheral blood lymphocytes

    • General notes

      Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Protein G purified

    • Purification notes

      ab33252 was purified from tissue culture supernatant.

    • Clonality

      Monoclonal

    • Clone number

      YTH862.2

    • Isotype

      IgG2b

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab33252 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/50 - 1/100.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.

     

     

     

    ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

    Target

    Images

    • Flow Cytometry - Anti-HLA Class I antibody [YTH862.2] (ab33252)
      Flow Cytometry - Anti-HLA Class I antibody [YTH862.2] (ab33252)
      FACS analysis of HLA class I expressing cells in human peripheral blood lymphocytes using ab33252.

    References (0)

    Publishing research using ab33252? Please let us know so that we can cite the reference in this datasheet.

    ab33252 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA Class I antibody [YTH862.2]

     Poor
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human xenograft in mouse)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    Yes - 0.1% Triton X
    Specification
    Human xenograft in mouse
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 18 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA Class I antibody [YTH862.2]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Infarcted Mouse Heart injected /w or w/o human mon)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Infarcted Mouse Heart injected /w or w/o human mon
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 20 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-HLA Class I antibody [YTH862.2]

     Average
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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