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I am searching for an antibody to HLA Class I that can be used for staining human cells injected into mouse heart.
Asked on Feb 22 2012
Thank you for your phone call. As we discussed, the best staining on mouse tissue will be obtained using a primary antibody raised in a species other than mouse in combination with a secondary antibody that has been pre-adsorbed to remove cross-reactivity with mouse (primary ab33252 with secondary ab98420 for example), or a directly conjugated primary antibody.
In addition to our conjugated primary antibodies to HLA Class I, if you are interested in any of our unconjugated mouse monoclonal antibodies you can either use one of our EasyLink conjugation kits to directly conjugate it (https://www.abcam.com/EasyLink), or pretreat your samples to avoid mouse-on-mouse staining problems. The pretreatment can be performed using a f(ab) fragment unconjugated antibody against mouse IgG such as ab6668. This can be performed at the same time as the serum block, for 1 hour at a concentration of 1:200-1:500.
for ab21148: DISCOUNT CODE: ***
for ab33252: DISCOUNT CODE: ***
Expiration date: June 22nd, 2012
I am very pleased to hear you would like to accept our offer and test ab21148 inIHC-Fr and ab33252 in mouse.Each code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview foreach antibodyand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.
Answered on Feb 22 2012