For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    hla-class-ii-drb1-antibody-da2-ab20184.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Blood Blood Cell Antigens
Share by email

Anti-HLA Class II DRB1 antibody [DA2] (ab20184)

  • Datasheet
Reviews (1) Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Mouse monoclonal [DA2] to HLA Class II DRB1
  • Suitable for: IHC-Fr, ELISA, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human HLA Class II DRB1 protein (denatured) (ab177593)
Primary
HRP Anti-HLA Class II DRB1 antibody (ab193646)
Primary
Product image
Anti-HLA-DR antibody [TAL 1B5] (ab20181)

View more associated products

Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [DA2]
    See all HLA Class II DRB1 primary antibodies
  • Description

    Mouse monoclonal [DA2] to HLA Class II DRB1
  • Host species

    Mouse
  • Specificity

    HLA-DR beta chain 1. All DR and DP except DR7. Not specific to humans.
  • Tested applications

    Suitable for: IHC-Fr, ELISA, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to HLA Class II DRB1. The membranes of LKT cells (a B lymphoblastoid cell line).

  • Epitope

    1st domain, monomorphic.
  • General notes

    Not cytotoxic.


    This antibody can be used for HLA typing.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Primary antibody notes

    This antibody can be used for HLA typing.
  • Clonality

    Monoclonal
  • Clone number

    DA2
  • Myeloma

    P3-x63-Ag8
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Adaptive Immunity
    • MHC
    • Class II

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human HLA Class II DRB1 protein (denatured) (ab177593)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ELISA (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.

Predicted molecular weight: 30 kDa (unprocessed precursor). Blots dimer and beta chain.

Notes
IHC-Fr
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.

Predicted molecular weight: 30 kDa (unprocessed precursor). Blots dimer and beta chain.

Target

  • Target information above from: UniProt accession P01911 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3123 Human
    • Omim: 142857 Human
    • SwissProt: P01911 Human
    • SwissProt: P01912 Human
    • SwissProt: P04229 Human
    • SwissProt: P20039 Human
    • SwissProt: Q29974 Human
    • SwissProt: Q30134 Human
    • SwissProt: Q30167 Human
    • SwissProt: Q5Y7A7 Human
    • SwissProt: Q95IE3 Human
    • SwissProt: Q9GIY3 Human
    • Unigene: 534322 Human
    • Unigene: 696211 Human
    • Unigene: 736560 Human
    see all
  • Alternative names

    • 2B1F_HUMAN antibody
    • DR1 antibody
    • DR16 antibody
    • DR5 antibody
    • DR8 antibody
    • DRB1 antibody
    • DRw10 antibody
    • DW2.2/DR2.2 antibody
    • FLJ75017 antibody
    • FLJ76359 antibody
    • HLA class II antigen beta chain antibody
    • HLA class II histocompatibility antigen, DR-1 beta chain antibody
    • HLA class II histocompatibility antigen, DRB1-15 beta chain antibody
    • HLA-DR-beta 1 antibody
    • HLA-DR1B antibody
    • HLA-DRB antibody
    • HLA-DRB1 antibody
    • human leucocyte antigen DRB1 antibody
    • Leucocyte antigen DR beta 1 chain antibody
    • leucocyte antigen DRB1 antibody
    • lymphocyte antigen DRB1 antibody
    • major histocompatibility complex, class II, DR beta 1 antibody
    • MHC class II antigen antibody
    • MHC class II antigen DRB1*15 antibody
    • MHC class II antigen HLA-DR13 antibody
    • MHC class II HLA-DR beta 1 chain antibody
    • MHC class II HLA-DR-beta cell surface glycoprotein antibody
    • MHC class II HLA-DRw10-beta antibody
    • SS1 antibody
    see all

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab20184? Please let us know so that we can cite the reference in this datasheet.

ab20184 has been referenced in 2 publications.

  • Brodsky FM  et al. Monoclonal antibodies to HLA--DRw determinants. Tissue Antigens 16:30-48 (1980). PubMed: 6162228
  • Brodsky FM  et al. Monoclonal antibodies for analysis of the HLA system. Immunol Rev 47:3-61 (1979). PubMed: 95015

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

ELISA abreview for Anti-HLA DR beta antibody [DA2]

Good
Abreviews
Abreviews
Application
ELISA
Sample
Human Recombinant protein (HLA DR52)
Specification
HLA DR52
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Type
Sandwich (Detection)
Read More

Abcam user community

Verified customer

Submitted Oct 07 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.