Anti-HLA Class II DRB1 antibody [DA2] (ab20184)
Key features and details
- Mouse monoclonal [DA2] to HLA Class II DRB1
- Suitable for: IHC-Fr, ELISA, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-HLA Class II DRB1 antibody [DA2]
See all HLA Class II DRB1 primary antibodies -
Description
Mouse monoclonal [DA2] to HLA Class II DRB1 -
Host species
Mouse -
Specificity
HLA-DR beta chain 1. All DR and DP except DR7. Not specific to humans. -
Tested applications
Suitable for: IHC-Fr, ELISA, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to HLA Class II DRB1. The membranes of LKT cells (a B lymphoblastoid cell line).
-
Epitope
1st domain, monomorphic. -
General notes
Not cytotoxic.This antibody can be used for HLA typing.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Primary antibody notes
This antibody can be used for HLA typing. -
Clonality
Monoclonal -
Clone number
DA2 -
Myeloma
P3-x63-Ag8 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
Predicted molecular weight: 30 kDa (unprocessed precursor). Blots dimer and beta chain. |
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. Predicted molecular weight: 30 kDa (unprocessed precursor). Blots dimer and beta chain. |
Target
- Information by UniProt
-
Database links
- Entrez Gene: 3123 Human
- Omim: 142857 Human
- SwissProt: P01911 Human
- SwissProt: P01912 Human
- SwissProt: P04229 Human
- SwissProt: P20039 Human
- SwissProt: Q29974 Human
- SwissProt: Q30134 Human
see all -
Alternative names
- 2B1F_HUMAN antibody
- DR1 antibody
- DR16 antibody
see all
Protocols
Datasheets and documents
-
Datasheet download
References (2)
ab20184 has been referenced in 2 publications.
- Brodsky FM et al. Monoclonal antibodies to HLA--DRw determinants. Tissue Antigens 16:30-48 (1980). PubMed: 6162228
- Brodsky FM et al. Monoclonal antibodies for analysis of the HLA system. Immunol Rev 47:3-61 (1979). PubMed: 95015
