Anti-HLA Class II DRB1 antibody [DA2] (ab20184)

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Key features and details

  • Mouse monoclonal [DA2] to HLA Class II DRB1
  • Suitable for: Flow Cyt, IHC-Fr, WB, ELISA
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [DA2]
    See all HLA Class II DRB1 primary antibodies

  • Description

    Mouse monoclonal [DA2] to HLA Class II DRB1

  • Host species

    Mouse

  • Specificity

    HLA-DR beta chain 1. All DR and DP except DR7. Not specific to humans.

  • Tested applications

    Suitable for: Flow Cyt, IHC-Fr, WB, ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to HLA Class II DRB1. Raised against the membranes of LKT cells of human origin.

  • Epitope

    1st domain, monomorphic.

  • General notes

    Not cytotoxic.


    This antibody can be used for HLA typing.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Primary antibody notes

    This antibody can be used for HLA typing.

  • Clonality

    Monoclonal

  • Clone number

    DA2

  • Myeloma

    P3-x63-Ag8

  • Isotype

    IgG1

  • Light chain type

    unknown

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.

Predicted molecular weight: 30 kDa (unprocessed precursor). Blots dimer and beta chain.
ELISA Use at an assay dependent concentration.

Target

References

Publishing research using ab20184? Please let us know so that we can cite the reference in this datasheet.

ab20184 has been referenced in 2 publications.

  • Brodsky FM  et al. Monoclonal antibodies to HLA--DRw determinants. Tissue Antigens 16:30-48 (1980). PubMed: 6162228
  • Brodsky FM  et al. Monoclonal antibodies for analysis of the HLA system. Immunol Rev 47:3-61 (1979). PubMed: 95015

Customer reviews and Q&As

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ELISA abreview for Anti-HLA DR beta antibody [DA2]

 Good
Abreviews
Application
ELISA
Sample
Human Recombinant protein (HLA DR52)
Specification
HLA DR52
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Type
Sandwich (Detection)
Read More

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Submitted Oct 07 2008

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